bs-0013R [Primary Antibody]
Cytochrome C Polyclonal Antibody
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Host: Rabbit

Target Protein: Cytochrome C

Immunogen Range: 51-105/105


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 54205

Swiss Prot: P99999

Source: KLH conjugated synthetic peptide derived from human Cytochrome C

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 12


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Shuhua, et al. "μ-Calpain mediates hippocampal neuron death in rats after lithium–pilocarpine-induced status epilepticus." Brain research bulletin 76.1 (2008): 90-96.Read more>>
  • Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014).Read more>>
  • Chen, Chengzhi, et al. "Resveratrol protects against arsenic trioxide‐induced oxidative damage through maintenance of glutathione homeostasis and inhibition of apoptotic progression." Environmental and Molecular Mutagenesis (2014).Read more>>
  • Gu, Shiyan, et al. "Resveratrol Synergistically Triggers Apoptotic Cell Death with Arsenic Trioxide via Oxidative Stress in Human Lung Adenocarcinoma A549 Cells." Biological trace element research (2014): 1-12.Read more>>
  • Fang C, Zhang J, Qi D, Fan X, Luo J, et al. (2014) Evodiamine Induces G2/M Arrest and Apoptosis via Mitochondrial and Endoplasmic Reticulum Pathways in H446 and H1688 Human Small-Cell Lung Cancer Cells. PLoS ONE 9(12): e115204. Read more>>
  • Gao, Guanmin, et al. "Poly (ADP‑ribose) polymerase‑and cytochrome c‑mediated apoptosis induces hepatocyte injury in a rat model of hyperammonia‑induced hepatic failure." Molecular Medicine Reports.Read more>>
  • Liao, Peng, et al. "Organellar proteome analyses of ricin toxin-treated HeLa cells." Toxicology and industrial health (2014): 0748233714549066.Read more>>
  • Li, Aihong, et al. "Increased Expression of Mitochondrial Inner-Membrane Protein Mpv17 After Intracerebral Hemorrhage in Adult Rats." Neurochemical Research (2015): 1-11.Read more>>
  • Chen, Chengzhi, et al. "Nuclear translocation of nuclear factor kappa B is regulated by G protein signaling pathway in arsenite‐induced apoptosis in HBE cell line." Environmental Toxicology (2015).Read more>>
  • Gu, Shiyan, et al. "ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction underlie apoptosis induced by resveratrol and arsenic trioxide in A549 cells." Chemico-Biological Interactions (2016).Read more>>
  • Duan, Xiaoxu, et al. "Antioxidant tert-butylhydroquinone ameliorates arsenic-induced intracellular damages and apoptosis through induction of Nrf2-dependent antioxidant responses as well as stabilization of anti-apoptotic factor Bcl− 2 in human keratinocytes." Free Radical Biology and Medicine(2016).Read more>>
  • Ye, Jinxia, et al. "Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca2."Evidence-Based Complementary and Alternative Medicine 2015 (2015).Read more>>
  • Liu, Yi, et al. "GluR3B Ab’s induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction." Brain Research Bulletin (2017).Read more>>
  • Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." Naunyn-Schmiedeberg's Archives of Pharmacology (2017): 1-17.Read more>>
  • Tian, Qin, et al. "Phosphoprotein Gene Contributes to the Enhanced Apoptosis Induced by Wild-Type Rabies Virus GD-SH-01 In Vitro." Frontiers in Microbiology 8 (2017): 1697.Read more>>
  • Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801Read more>>
  • Zhang et al. Sulfiredoxin-1 protects against simulated ischaemia/reperfusion injury in cardiomyocyte by inhibiting PI3K/AKT-regulated mitochondrial apoptotic pathways. (2016) Biosci.Rep. 36 Read more>>
  • Song et al. Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. (2018) Exp.Ther.Med. 15:5251-5260Read more>>
  • Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. Read more>>
  • Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. Read more>>
  • Wang Y et al. Excessive Cu2+ deteriorates arsenite-induced apoptosis in chicken brain and resulting in immunosuppression, not in homeostasis. Chemosphere. 2019 Sep 4;239:124758. Read more>>
  • Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583. Read more>>
  • Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583. Read more>>
  • Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27. Read more>>
  • Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245. Read more>>
  • Shao Y et al. Copper-Mediated Mitochondrial Fission/Fusion Is Associated with Intrinsic Apoptosis and Autophagy in the Testis Tissues of Chicken.Biol Trace Elem Res. 2018 Jul 4. Read more>>
  • Guo MB et al. Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells.Eur Spine J. 2018 Oct;27(10):2609-2620. Read more>>
  • Wu Z et al. Compound xiebai capsule alleviates pulmonary vascular remodeling in monocrotaline-induced pulmonary arterial hypertension in rats. Tropical Journal of Pharmaceutical Research January 2020; 19 (1): 107-114.Read more>>
  • Rui X et al. Imperative and effective reversion of synovial hyperplasia and cartilage destruction in rheumatoid arthritis through multiple synergistic effects of O2 and Ca2+. Mater Sci Eng C Mater Biol Appl.2020 Sep;114:111058.Read more>>
  • Dasari S et al. Neferine, an alkaloid from lotus seed embryo targets HeLa and SiHa cervical cancer cells via pro\oxidant anticancer mechanism. Phytother Res. 2020 Sep;34(9):2366-2384.Read more>>
  • Li Y et al. Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens. Mol Cell Proteomics. 2020 Jun;19(6):1035-1046.Read more>>
  • Shuli Shao. et al. Toosendanin induces apoptosis of MKN?45 human gastric cancer cells partly through miR?23a?3p?mediated downregulation of BCL2. Mol Med Rep. 2020 Sep;22(3):1793-1802Read more>>
  • Jiarong Yanget al. Effects of Different Doses of Calcium on the Mitochondrial Apoptotic Pathway and Rho/ROCK Signaling Pathway in the Bone of Fluorosis Rats. Biol Trace Elem Res. 2020 Jul 25.Read more>>
  • Zhang Y et al. Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat modelPhytother Res.2020 Dec 10.Read more>>
  • Lei Tian. et al. Acute ozone exposure can cause cardiotoxicity: Mitochondria play an important role in mediating myocardial apoptosis. Chemosphere. 2021 Apr;268:128838Read more>>
  • Hanting Xu. et al. Melatonin alleviates benzo(a)pyrene-induced ovarian corpus luteum dysfunction by suppressing excessive oxidative stress and apoptosis. Ecotox Environ Safe. 2021 Jan;207:111561Read more>>
  • Yajing Wang. et al. Long-term exposure to the fluoride blocks the development of chondrocytes in the ducks: The molecular mechanism of fluoride regulating autophagy and apoptosis. Ecotox Environ Safe. 2021 Jul;217:112225Read more>>
  • Shaofeng Wu. et al. Protective effects of curcumin on ATO-induced nephrotoxicity in ducks in relation to suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotox Environ Safe. 2021 Aug;219:11235Read more>>
  • Liang Lyu. et al. Autophagy inhibition enhances anti-pituitary adenoma effect of tetrandrine. 2021 May 26Read more>>
  • Shaofeng Wu. et al. Evaluation of toxic effects induced by arsenic trioxide or/and antimony on autophagy and apoptosis in testis of adult mice. 2021 May 2Read more>>
  • Jingxue Ma. et al. Depletion of Fractalkine ameliorates renal injury and Treg cell apoptosis via the p38MAPK pathway in lupus-prone mice. Exp Cell Res. 2021 Aug;405:112704Read more>>
  • Guangcong Peng. et al. Intranasal administration of DHED protects against exhaustive exercise-induced brain injury in rats. Brain Res. 2021 Dec;1772:147665Read more>>
  • Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11Read more>>
  • Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078Read more>>
  • Rui Feng. et al. Progesterone regulates inflammation and receptivity of cells via the NF-_B and LIF/STAT3 pathways. THERIOGENOLOGY. Theriogenology. 2022 Jul;186:5Read more>>
  • Hu, Zhuoying. et al. Effects of Long-Term Exposure to Copper on Mitochondria-Mediated Apoptosis in Pig Liver. BIOL TRACE ELEM RES. 2022 Jun;:1-14Read more>>
  • Xu Kejia. et al. Anti-melanoma effect and action mechanism of a novel chitosan-based composite hydrogel containing hydroxyapatite nanoparticles. REGEN BIOMATER. 2022 JulRead more>>
  • Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186Read more>>
  • Wenyu Guo. et al. Ultrasound-Mediated Antitumor Therapy via Targeted Acoustic Release Carrier of Carbon Monoxide (TARC-CO). ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Li, Guang. et al. Synergetic delivery of artesunate and isosorbide 5-mononitrate with reduction-sensitive polymer nanoparticles for ovarian cancer chemotherapy. J NANOBIOTECHNOL. 2022 Dec;20(1):1-13Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining


Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature


HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).


Tissue/cell:Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cytochrome C) polyclonal Antibody, Unconjugated (bs-0013R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


25 ug total protein per lane of various lysates (see on figure) probed with Cytochrome C polyclonal antibody, unconjugated (bs-0013R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.