bs-0014R [Primary Antibody]
IGF 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IGF 1

Immunogen Range: 49-100/195

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3479

Swiss Prot: P05019

Source: KLH conjugated synthetic peptide derived from human IGF-1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)

Cross Reactive Species: Human
Mouse
Rat
Dog

Predicted Cross Reactive Species: Cow
Sheep
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Baykara, B., et al. "Progesterone treatment decreases traumatic brain injury induced anxiety and is correlated with increased serum IGF-1 levels; prefrontal cortex, amygdala, hippocampus neuron density; and reduced serum corticosterone levels in immature rats." Biotechnic & Histochemistry 0 (2013): 1-8.Read more>>
  • Kiray, M., et al. "Effects of carbon dioxide exposure on early brain development in rats." Biotechnic & Histochemistry 0 (2014): 1-13.Read more>>
  • Gabr, Hala, et al. "The effect of bone marrow‐derived mesenchymal stem cells on chemotherapy induced ovarian failure in albino rats." Microscopy Research and Technique (2016).Read more>>
  • Liao, Wei-Tao, et al. "The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation." (2016).Read more>>
  • Müller, L., et al. "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." Reproduction in Domestic Animals (2017).Read more>>
  • Uysal, Nazan, et al. "Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats." Journal of Chemical Neuroanatomy (2017).Read more>>
  • Liao et al. The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation. (2018) Arch.Med.Sci. 14:370-380Read more>>
  • Muller et al. Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs. (2017) Reprod.Domest.Anim. 52 Suppl 2:187-192Read more>>
  • Zhao L et al. Reducing macrophage numbers alleviates temporomandibular joint ankylosis. Cell Tissue Res. 2019 Sep 14.Read more>>
  • Li R et al. Pharmacological biotargets and the molecular mechanisms of oxyresveratrol treating colorectal cancer: Network and experimental analyses. Biofactors. 2019 Oct 24.Read more>>
  • Yokohira M et al. Lung proliferative lesion‐promoting effects of left pulmonary ligation in A/J female mice. Pathol Int. 2020 Feb 21. Read more>>
  • Ito E et al. Food restriction reduces cortical bone mass and serum insulin-like growth factor-1 levels and promotes uterine atrophy in miceBiochem Biophys Res Commun.2021 Jan 1;534:165-171.Read more>>
VALIDATION IMAGES

Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.


Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4°C, DAB staining.