bs-0016R [Primary Antibody]
Tyrosine Hydroxylase Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Tyrosine Hydroxylase

Immunogen Range: 101-165/528


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7054

Swiss Prot: P07101

Source: KLH conjugated synthetic peptide derived from human Tyrosine Hydroxylase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Neuronal Marker Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 60


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu, Yaping, et al. "Overexpression of lactate dehydrogenase-A in human intrahepatic cholangiocarcinoma: its implication for treatment." World Journal of Surgical Oncology 12.1 (2014): 78.Read more>>
  • Shu et al. Connexin 43 is involved in the sympathetic atrial fibrillation in canine and canine atrial myocytes. (2017) Anatol.J.Cardiol. 18:3-9Read more>>
  • Yue Y et al. Noradrenaline-Specific, Efficient Visualization in Brain Tissue Triggered by Unique Cascade Nucleophilic Substitution. Anal Chem. 2019 Feb 5;91(3):2255-2259.Read more>>
  • Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25. Read more>>
  • Ozbey G et al. Metformin protects rotenone-induced dopaminergic neurodegeneration by reducing lipid peroxidation. Pharmacol Rep. 2020 Mar 23. Read more>>
  • Na Zhou. et al. Specific Fluorescent Probe Based on ProtectCDeprotect To Visualize the Norepinephrine Signaling Pathway and Drug Intervention Tracers. J Am Chem Soc. 2020;142(41):17751C17755Read more>>
VALIDATION IMAGES

Human PC-3 lysates probed with Tyrosine Hydroxylase Polyclonal Antibody, unconjugated (bs-0016R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Tyrosine Hydroxylase(Neuronal Marker)/Tyk2/TYH Polyclonal Antibody, Unconjugated (bs-0016R) 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 1:200 overnight at 4°C, DAB staining.