bs-0027R
[Primary Antibody]
Melatonin Receptor 1A Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Melatonin Receptor 1A
Immunogen Range: 201-280/350
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4543
Swiss Prot: P48039
Source: KLH conjugated synthetic peptide derived from human MTR-1A
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
dot-blot()
Predicted Molecular Weight: 39
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Others
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Chicken
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, S-J., et al. "Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)." Theriogenology 78.7 (2012): 1517-1526.Read more>>
- Tabecka-Lonczyska, Anna, et al. "New insight on the role of melatonin receptors in reproductive processes of seasonal breeders on the example of mature male European bison (Bison bonasus, Linnaeus 1758)." Journal of Photochemistry and Photobiology B: Biology (2017).Read more>>
- Jang, Hoon, et al. "Synergistic effect of melatonin and ghrelin in preventing cisplatin‐induced ovarian damage via regulation of FOXO3a phosphorylation and binding to the p27Kip1 promoter in primordial follicles." Journal of Pineal Research (2017).Read more>>
- Yang, Wucai, et al. "Melatonin promotes triacylglycerol accumulation via MT2 receptor during differentiation in bovine intramuscular preadipocytes." Scientific Reports 7.1 (2017): 15080.Read more>>
- Han D et al. Activation of Melatonin Receptor 2 But Not Melatonin Receptor 1 Mediates Melatonin‐conferred Cardio‐protection Against Myocardial Ischemia/Reperfusion Injury.J Pineal Res. 2019 Mar 22:e12571.Read more>>
- Gao Y et al. Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells.Cell Mol Biol Lett. 2019 Mar 12;24:21.Read more>>
- Zhao Shu-Qin. et al. The in vitro effects of melatonin and Cry gene on the secretion of estradiol from camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jan;74:106497Read more>>
- S-Q. Zhao. et al. cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jul;76:106609Read more>>
- Dejun Xu. et al. Melatonin protects mouse testes from palmitic acid\induced lipotoxicity by attenuating oxidative stress and DNA damage in a SIRT1\dependent manner. J Pineal Res. 2020 Nov;69(4):e1269Read more>>
- Donghui Yang. et al. Melatonin alleviates LPS\induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551Read more>>
- Shanshan Yu. et al. Melatonin prevents experimental central serous chorioretinopathy in rats. J PINEAL RES. 2022 Apr 29Read more>>
- Tang Qian. et al. The Effect of Melatonin on Radicular Pain in a Rat Model of Lumbar Disc Herniation. SPINE. 2022 May;47(10):754-763Read more>>
- Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 AugRead more>>
- Hongwei Duan. et al. The effect of melatonin on sheep endometrial epithelial cell apoptosis through the receptor and non-receptor pathways. GEN COMP ENDOCR. 2022 Nov;:114182Read more>>
- Hongwei Duan. et al. Melatonin regulates dihydrotestosterone formation via its membrane receptor in the epididymal epithelial cells of sheep. THERIOGENOLOGY. 2022 DecRead more>>
- Junyuan Ma. et al. Effects of melatonin on development and hormone secretion of sheep theca cells in?vitro. THERIOGENOLOGY. 2023 Mar;198:172Read more>>
- G?k?e Nur Ar?k. et al. Melatonin effective to reduce the microscopic symptoms of polycystic ovary syndrome-related infertility: An experimental study. TISSUE CELL. 2023 Jan;:102015Read more>>
- Arzu Yalcin. et al. Protective effect of melatonin on learning and memory impairment and hippocampal dysfunction in rats induced by high-fructose corn syrup. IRAN J BASIC MED SCI. 2023 Jan;26(1):69-75Read more>>
- Sanghoon Lee. et al. Expression of the melatonergic system during meiotic maturation of cynomolgus monkey cumulusCoocyte complexes. J MED PRIMATOL. 2023 MaRead more>>
- Gao Xinran. et al. Role of Bmal1 in Type 2 Diabetes Mellitus-Related Glycolipid Metabolic Disorder and Neuropsychiatric Injury: Involved in the Regulation of Synaptic Plasticity and Circadian Rhythms. MOL NEUROBIOL. 2023 May;:1-23Read more>>
- Chia-Hsuan Chang. et al. Modulatory effect of n-3 polyunsaturated fatty acids on depressive-like behaviors in rats with chronic sleep deprivation: potential involvement of melatonin receptor pathway and brain lipidome. FOOD FUNCT. 2023 Jul;14(13):5977-5993Read more>>
- ingcong Li. et al. Melatonin Ameliorates Neuropsychiatric Behaviors, Gut Microbiome, and Microbiota-Derived Metabolites in Rats with Chronic Sleep Deprivation. INT J MOL SCI. 2023 Jan;24(23):1682Read more>>
- Hongwei Duan. et al. Melatonin promotes progesterone secretion in sheep luteal cells by regulating autophagy via the AMPK/mTOR pathway. THERIOGENOLOGY. 2023 NovRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-MTNR1A/MTR-1A/MEL-1A-R Polyclonal Antibody, Unconjugated (bs-0027R) at 1:300 followed by conjugation to the secondary antibody
Lane 1: Cerebrum lysates probed with Melatonin Receptor 1A Antibody (bs-0027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Melatonin Receptor 1A Polyclonal Antibody(bs-0027R) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.