bs-0033R-A350 [Conjugated Primary Antibody]
P53 protein(wt-p53) Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P53 proteinwt-p53

Immunogen Range: 251-310/393

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7157

Swiss Prot: P04637

Source: KLH conjugated synthetic peptide derived from human P53

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at 4°C for 12 months.

Background: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 343nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 43

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.Read more>>
  • Wang, Chao, et al. "Hypoxia inhibits myogenic differentiation through p53-dependent induction of Bhlhe40." Journal of Biological Chemistry (2015): jbc-M115.Read more>>
  • Hong, Haizheng, et al. "Developmental toxicity of three hexabromocyclododecane diastereoisomers in embryos of the marine medaka Oryzias melastigma." Marine pollution bulletin (2015).Read more>>
  • Celik‐Ozenci, C., et al. "Inhibition of poly (ADP‐ribose) polymerase may have preventive potential for varicocoele‐associated testicular damage in rats." Andrology (2016).Read more>>
  • Sukohar, Asep, and Mohammad Kanedi. "Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and P53, in T47D Breast Cancer Cells." Biomedical and Pharmacology Journal 10.2 (2017): 641-649.Read more>>
  • Uchiumi et al. Characterization of the 5'-flanking region of the human TP53 gene and its response to the natural compound, Resveratrol. (2016) J.Bioche. 159:437-47Read more>>
  • Celik-Ozenci et al. Inhibition of poly(ADP-ribose) polymerase may have preventive potential for varicocoele-associated testicular damage in rats. (2017) Andrology. 5:362-369Read more>>
  • Liu F et al. Bioinformatic and experimental findings to indicate anti-cancer targets and mechanisms of calycosin against nasopharyngeal carcinoma. Research Square;12 February 2020.Read more>>
  • Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
  • Tan J et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020 Jun;126:110096.Read more>>
  • Liu F et al. Anticancer targets and mechanisms of calycosin to treat nasopharyngeal carcinoma. Biofactors. 2020 Jul;46(4):675-684.Read more>>
  • Wenlan Yu. et al. Chronic tribasic copper chloride exposure induces rat liver damage by disrupting the mitophagy and apoptosis pathways. Ecotox Environ Safe. 2021 Apr;212:111968Read more>>
  • Yunping Lu. et al. Peroxiredoxin1 Knockdown Inhibits Oral Carcinogenesis via Inducing Cell Senescence Dependent on Mitophagy. Oncotargets Ther. 2021; 14: 239C251Read more>>
  • Ling-Chu Hung. et al. The Monoclonal Antibody Recognized the Open Reading Frame Protein in Porcine Circovirus Type 2-Infected Peripheral Blood Mononuclear Cells. Viruses-Basel. 2020 Sep;12(9):961Read more>>
  • Yajing Wang. et al. Long-term exposure to the fluoride blocks the development of chondrocytes in the ducks: The molecular mechanism of fluoride regulating autophagy and apoptosis. Ecotox Environ Safe. 2021 Jul;217:112225Read more>>
VALIDATION IMAGES