bs-0035R [Primary Antibody]
CREB-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CREB-1

Immunogen Range: 101-150/341

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1385

Swiss Prot: P16220

Source: KLH conjugated synthetic peptide derived from human CREB-1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 37

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang et al. Neuroprotective effect of arctigenin via upregulation of P-CREB in mouse primary neurons and human SH-SY5Y neuroblastoma cells. (2013) Int.J.Mol.Sci. 14:18657-69Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human cervial carcinoma labeled with Anti-CREB-1 Polyclonal Antibody, Unconjugated (bs-0035R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: HeLa lysates probed with Rabbit Anti-CREB-1 Polyclonal Antibody, Unconjugated (bs-0035R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CREB-1 Polyclonal Antibody, Unconjugated (bs-0035R) at 1:200 overnight at 4°C, DAB staining.