bs-0036R [Primary Antibody]
CREB-1(Ser133) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CREB-1 Ser133

Modification Site: Ser133

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1385

Swiss Prot: P16220

Source: KLH conjugated synthetic phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser133 [RP(p-S)YR]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Sheep
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu et al. Cardioprotection against experimental myocardial ischemic injury using cornin. (2016) Braz.J.Med.Biol.Re. 49:e5039Read more>>
  • Ding H et al. Expression and regulation of GnRHR2 gene and testosterone secretion mediated by GnRH2 and GnRHR2 within porcine testes. J Steroid Biochem Mol Biol. 2019 Jun;190:161-172. Read more>>
  • S-Q. Zhao. et al. cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jul;76:106609Read more>>
  • Zhenting Huang . et al. Anti-Cognitive Decline by Yinxing-Mihuan-Oral-Liquid via Activating CREB/BDNF Signaling and Inhibiting Neuroinflammatory Process. 2021 Jan 27Read more>>
  • ei Miao. et al. Protective effect of HCN2-induced SON sensitization on chronic visceral hypersensitivity in neonatal-CRD rat model. Brain Res. 2021 Sep;1767:147538Read more>>
  • Zhixia Jia. et al. Baicalin Ameliorates Chronic Unpredictable Mild Stress-Induced Depression through the BDNF/ERK/CREB Signaling Pathway. Behav Brain Res. 2021 Jul;:113463Read more>>
  • He Zhang. et al. Effect of feeding regimen on circadian activity rhythms of food anticipatory by ghrelin hormone in a pig model. 2022 Mar 05Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody (bs-0036R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse hippocampus labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody


Formalin-fixed and paraffin-embedded rat brain labeled with Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated(bs-0036R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Rat RSC96 cells probed with CREB1 (Ser133) Polyclonal Antibody, Unconjugated (bs-0036R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).


Mouse spleen lysate probed with Anti-CREB-1(Ser133) Polyclonal Antibody (bs-0036R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Mouse Kidney lysates; Lane 4: Human HUVEC cell lysates probed with CREB-1 Polyclonal Antibody, Unconjugated (bs-0036R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.