bs-0037R [Primary Antibody]
AIF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AIF

Immunogen Range: 131-230/613

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9131

Swiss Prot: O95831

Source: KLH conjugated synthetic peptide derived from human AIF

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Sheep
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gong, Ping, et al. "Hypothermia-induced neuroprotection is associated with reduced mitochondrial membrane permeability in a swine model of cardiac arrest." Journal of Cerebral Blood Flow & Metabolism (2013).Read more>>
  • Li, Zhiping, et al. "Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer’s Disease Mouse Model." International Journal of Molecular Sciences 18.8 (2017): 1623.Read more>>
  • Ba et al. Effect of cilostazol pretreatment on the PARP/AIF-mediated apoptotic pathway in rat cerebral ischemia-reperfusion models. (2014) Exp.Ther.Me. 7:1209-1214Read more>>
  • Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. Read more>>
  • Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583. Read more>>
  • Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27. Read more>>
  • Zhang Y et al. Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat modelPhytother Res.2020 Dec 10.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF Polyclonal Antibody, Unconjugated (bs-0037R) 1:300 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti-AIF Polyclonal Antibody, Unconjugated (bs-0037R) 1:300 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE (Negative Control)


Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti-AIF Polyclonal Antibody, Unconjugated (bs-0037R) 1:300 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE


Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-AIF Polyclonal Antibody, Unconjugated (bs-0037R), followed by conjugation to the secondary antibody and DAB staining


K562 lysates probed with AIF Polyclonal Antibody, unconjugated (bs-0037R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Mouse Liver lysates probed with AIF Polyclonal Antibody, unconjugated (bs-0037R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Lane 1: K562 cell lysates; Lane 2: Rat Testis lysates; Lane 3: Mouse Testis lysates probed with AIF Polyclonal Antibody, Unconjugated (bs-0037R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.