Host: Rabbit
Target Protein: Insulin
Immunogen Range: 46-59/110
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3630
Swiss Prot: P01308
Source: KLH conjugated synthetic peptide derived from human Insulin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 5.8/12
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Yang et al. Paradoxical effect of rapamycin on inflammatory stress-induced insulin resistance in vitro and in vivo. (2015) Sci.Re. 5:14959Read more>>
- Xinghao Jiang. et al. Fibroblast growth factor 21: a novel long-acting hypoglycemic drug for canine diabetes. 2020 Nov 2Read more>>
- Yu Fan. et al. The role of substance P in acupuncture signal transduction and effects. Brain Behav Immun. 2021 Jan;91:683Read more>>
- Xiaohang Wang. et al. Screening and Identification of Key Genes for Activation of Islet Stellate Cell. Front Endocrinol. 2021; 12: 695467Read more>>
- Lagunas-Rangel Francisco Alejandro. et al. Triple drug therapy with GABA, sitagliptin, and omeprazole prevents type 1 diabetes onset and promotes its reversal in non-obese diabetic mice. FRONT ENDOCRINOL. 2022 Oct;0:2702Read more>>
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Insulin Polyclonal Antibody, Unconjugated(bs-0056R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-Insulin Polyclonal Antibody, Unconjugated(bs-0056R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.
Mouse Pancreas (C57BL/6 strain) fixed in 4% PFA for 2 hours at RT, then transferred to 30% sucrose overnight at 4°C before embedding in OCT mounting medium. Treated with TBS containing 5% goat serum and 0.3% Triton X-100, to block unspecific binding sites and permeabilize cell membranes, for 30min at RT. Antibody (bs-0056R-FITC) diluted at 1:100 in TBS containing 2% goat serum applied and incubated overnight at 4°C. Rinse sections in TBS 2x5min on a shaker, mount section with vectorshield (with DAPI), then cover them with coverslips and seal the edge of the coverslip on the slide with nail polish. This image was kindly submitted by an end-user from the National Children's Research Centre, Our Lady's Children's Hospital, Dublin, Ireland.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Insulin Polyclonal Antibody, Unconjugated (bs-0056R) at 1:200 overnight at 4°C, DAB staining.