Host: Rabbit
Target Protein: APAF1CT
Immunogen Range: 1201-1248/1248
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 317
Swiss Prot: O14727
Source: KLH conjugated synthetic peptide derived from human Apaf-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 137
Cross Reactive Species:
Human
Rat
Zebrafish
Predicted Cross Reactive Species:
Mouse
Dog
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Shi et al. Human interleukin 23 receptor induces cell apoptosis in mammalian cells by intrinsic mitochondrial pathway associated with the down-regulation of RAS/mitogen-activated protein kinase and signal transducers and activators of transcription factor 3 signaling pathways. (2013) Int.J.Mol.Sc. 14:24656-69Read more>>
- Xiang Y et al. MiR-186 bidirectionally regulates cisplatin sensitivity of ovarian cancer cells via suppressing targets PIK3R3 and PTEN and upregulating APAF1 expression. J Cancer. 2020 Mar 13;11(12):3446-3453.Read more>>
- Qiuxiang Cai. et al. Pasteurella multocida causes liver injury in ducks by mediating inflammatory, apoptotic and autophagic pathways. MICROB PATHOGENESIS. 2023 Sep;:106336Read more>>
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti APAF1 Polyclonal Antibody, Unconjugated (bs-0058R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (APAF1(CT)) polyclonal Antibody, Unconjugated (bs-0058R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.