bs-0061R-A555 [Conjugated Primary Antibody]
beta Actin Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: beta-Actin

Immunogen Range: 1-200/375

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 60

Swiss Prot: P60709

Source: MAP conjugated synthetic peptide derived from human beta Actin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at 4°C for 12 months.

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Conjugation: ALEXA FLUOR® 555

Excitation/ Emission: 553nm/568nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Sheep
Bee
Chicken
Cat
Rabbit
Guinea Pig
Fish
Hamster

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han, Jianfeng, et al. "Cationic Bovine Serum Albumin Based Self‐Assembled Nanoparticles as siRNA Delivery Vector for Treating Lung Metastatic Cancer." Small 10.3 (2014): 524-535.Read more>>
  • Teng, I., et al. "Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer." Biomaterials (2013).Read more>>
  • Xiang, Bai, et al. "PSA-responsive and PSMA-mediated multifunctional liposomes for targeted therapy of prostate cancer." Biomaterials (2013).Read more>>
  • Li, Xiaoxia, et al. "AMP-activated protein kinase promotes the differentiation of endothelial progenitor cells." Arteriosclerosis, thrombosis, and vascular biology 28.10 (2008): 1789-1795.Read more>>
  • Ma, Yanqin, et al. "Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit aorta." Archives of toxicology 86.6 (2012): 849-856.Read more>>
  • Zeng, Xiaoyun, et al. "A Newly Synthesized Sinapic Acid Derivative Inhibits Endothelial Activation In Vitro and In Vivo." Molecular Pharmacology (2013).Read more>>
  • Wang, Ye-Jun, et al. "Two oral HBx vaccines delivered by live attenuated< i> Salmonella</i>: Both eliciting effective anti-tumor immunity." Cancer letters 263.1 (2008): 67-76.Read more>>
  • Fu, Chenglai, et al. "Cholesterol increases adhesion of monocytes to endothelium by moving adhesion molecules out of caveolae." Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids 1801.7 (2010): 702-710.Read more>>
  • Yin, Di, et al. "Functional graphene oxide as a plasmid-based Stat3 siRNA carrier inhibits mouse malignant melanoma growth in vivo." Nanotechnology 24.10 (2013): 105102.Read more>>
  • Yin, Di, et al. "Functional graphene oxide as a plasmid-based Stat3 siRNA carrier inhibits mouse malignant melanoma growth in vivo." Nanotechnology 24.10 (2013): 105102.Read more>>
  • Wang, Wei, et al. "Cynoglossus semilaevis ISG15: A Secreted Cytokine-Like Protein That Stimulates Antiviral Immune Response in a LRGG Motif-Dependent Manner." PLOS ONE 7.9 (2012): e44884.Read more>>
  • Gao, Jin-Hang, et al. "Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis." PLOS ONE 8.7 (2013): e69309.Read more>>
  • Hou, Cong-Cong, and Wan-Xi Yang. "Acroframosome-Dependent KIFC1 Facilitates Acrosome Formation during Spermatogenesis in the Caridean Shrimp Exopalaemon modestus."?PLOS ONE?8.9 (2013): e76065.Read more>>
  • Shi, Ran-ran, et al. "The immunogenicity of a novel cytotoxic T lymphocyte epitope from tumor antigen PL2L60 could be enhanced by 4-chlorophenylalanine substitution at position 1."?Cancer Immunology, Immunotherapy?(2013): 1-10.Read more>>
  • Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012).Read more>>
  • Li, Bing-Hua, et al. "Polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via inhibition CD95 pathway and reactive oxygen species." Free radical research 41.11 (2007): 1224-1232.Read more>>
  • Chen, Cheng, et al. "The galectin-3-binding protein of< i> Cynoglossus semilaevis</i> is a secreted protein of the innate immune system that binds a wide range of bacteria and is involved in host phagocytosis." Developmental & Comparative Immunology (2012).Read more>>
  • Qiu, Reng, et al. "Macrophage migration inhibitory factor of< i> Sciaenops ocellatus</i> regulates immune cell trafficking and is involved in pathogen-induced immune response." Developmental & Comparative Immunology (2013).Read more>>
  • Zhang, Jian, Ling Chen, and Li Sun. "SmLMWPTP, a teleost low molecular weight protein tyrosine phosphatase, inhibits the immune response of peripheral blood leukocytes in a manner that depends on the conserved P-loop." Developmental & Comparative Immunology (2013).Read more>>
  • Li, Mo-fei, et al. "First characterization of a teleost Epstein-Barr virus-induced gene 3 (EBI3) reveals a regulatory effect of EBI3 on the innate immune response of peripheral blood leukocytes." Developmental & Comparative Immunology (2013).Read more>>
  • Chen, Cheng, et al. "The galectin-3-binding protein of< i> Cynoglossus semilaevis</i> is a secreted protein of the innate immune system that binds a wide range of bacteria and is involved in host phagocytosis." Developmental & Comparative Immunology (2012).Read more>>
  • Liu, Ping, et al. "Effects of pubertal fenvalerate exposure on testosterone and estradiol synthesis and the expression of androgen and estrogen receptors in the developing brain." Toxicology Letters 201.2 (2011): 181-189.Read more>>
  • Ye, R., et al. "Ginsenoside Rd attenuates mitochondrial dysfunction and sequential apoptosis after transient focal ischemia." Neuroscience 178 (2011): 169-180.Read more>>
  • Liu, Donghui, et al. "Nonenzymatic glycation of high?\density lipoprotein impairs its anti?\inflammatory effects in innate immunity."iabetes/Metabolism Research and Reviews 28.2 (2012): 186-195..3Read more>>
  • Sun, Yuan, et al. "Carbon disulfide exposure at peri-implantation disrupts embryo implantation by decreasing integrin ??< sub> 3</sub> expression in the uterine tissue of pregnant mice."Chemico-biological interactions?(2013).xRead more>>
  • Yu, Lan-ping, et al. "Characterization of a c-type lysozyme of Scophthalmus maximus: expression, activity, and antibacterial effect." Fish & Shellfish Immunology (2012).Read more>>
  • Zhong, Zhiwei, et al. "miR-21 induces cell cycle at S phase and modulates cell proliferation by down-regulating hMSH2 in lung cancer." Journal of cancer research and clinical oncology 138.10 (2012): 1781-1788.Read more>>
  • Ye, Ruidong, et al. "Ginsenoside Rd attenuates early oxidative damage and sequential inflammatory response after transient focal ischemia in rats." Neurochemistry international 58.3 (2011): 391-398.Read more>>
  • Han, Xiaohua, et al. "Antioxidant action of 7, 8-dihydroxyflavone protects PC12 cells against 6-hydroxydopamine-induced cytotoxicity." Neurochemistry International (2013).Read more>>
  • Feng, Ming, et al. "Ulinastatin Attenuates Experimental Autoimmune Encephalomyelitis by Enhancing Anti-inflammatory Responses." Neurochemistry international (2013).Read more>>
  • Zhong, Zhiwei, et al. "MicroRNA-31-5p modulates cell cycle by targeting human mutL homolog 1 in human cancer cells." Tumor Biology (2013): 1-7.Read more>>
  • He, Siyi, et al. "miR-138 protects cardiomyocytes from hypoxia-induced apoptosis via MLK3/JNK/c-jun pathway." Biochemical and biophysical research communications (2013).Read more>>
  • Pan, Lin, et al. "Lin-28 reactivation is required for let-7 repression and proliferation in human small cell lung cancer cells." Molecular and cellular biochemistry 355.1-2 (2011): 257-263.Read more>>
  • Deng, Xiao Li, et al. "Comparative study on circulating endothelial progenitor cells in systemic lupus erythematosus patients at active stage." Rheumatology international 30.11 (2010): 1429-1436.Read more>>
  • Gao, Wei, et al. "Study of critical role of c-Met and its inhibitor SU11274 in colorectal carcinoma." Medical Oncology 30.2 (2013): 1-8.Read more>>
  • Zhang, Zhan, et al. "Differential expression of beta-catenin and dickkopf-1 in the third trimester placentas from normal and preeclamptic pregnancies: a comparative study." Reproductive Biology and Endocrinology 11.1 (2013): 17.Read more>>
  • Ruan Lin, et al. "Tetrandrine Attenuated Cerebral Ischemia/Reperfusion Injury and Induced Differential Proteomic Changes in a MCAO Mice Model Using 2-D DIGE." Neurochemical Research (2013). 1-9.Read more>>
  • Tao, Liu, et al. "In vitro study on human cytomegalovirus affecting early pregnancy villous EVT??s invasion function."Virology Journal 8.1 (2011): 1-8.DRead more>>
  • Nie, Lihong, et al. "The anti-apoptotic effect of hydrogen sulfide attenuates injuries to the medullary respiratory centers of neonatal rats subjected to in utero cigarette smoke exposure." Respiratory physiology & neurobiology (2013).Read more>>
  • Chen, Cui-Ying, et al. "Characterization of cytotoxicity-related gene expression in response to virulent Marek??s disease virus infection in the bursa of Fabricius."Research in veterinary science (2012).qRead more>>
  • Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013).Read more>>
  • GAO, DAN, et al. "Effect of peritoneal dialysis on expression of vascular endothelial growth factor, basic fibroblast growth factor and endostatin of the peritoneum in peritoneal dialysis patients." Nephrology 16.8 (2011): 736-742.Read more>>
  • Wang, Chunqiang, Wei Ma, and Yuhong Su. "NF-??B Pathway Contributes to Cadmium-Induced Apoptosis of Porcine Granulosa Cells."Biological trace element research (2013): 1-8.cRead more>>
  • Zhao, Yu, and Shijun Wang. "Neural Stem Cell Transplantation Inhibits Apoptosis through Activation of MAPK/ERK Signaling Pathway in Cerebral Ischemia/Reperfusion Rats." International Journal of Clinical and Experimental Neurology 1.1 (2013): 5-11.Read more>>
  • Zhou, Jing, et al. "Effects of low-dose hydroxychloroquine on expression of phosphorylated Akt and p53 proteins and cardiomyocyte apoptosis in peri-infarct myocardium in rats."?Experimental & Clinical Cardiology?18.2 (2013): e95.Read more>>
  • Zhang, Xiao-ling, et al. "Vascular endothelial growth factor up-regulates the expression of intracellular adhesion molecule-1 in retinal endothelial cells via reactive oxygen species, but not nitric oxide." Chinese Medical Journal (English Edition) 122.3 (2009): 338.Read more>>
  • Wang, Gui-Jun, et al. "Expression of tissue factor pathway inhibitor-2 in gastric stromal tumor and its clinical significance." Experimental and Therapeutic Medicine.Read more>>
  • Xu, R., et al. "Secretoneurin Induces Airway Mucus Hypersecretion by Enhancing the Binding of EGF to NRP1." Cellular Physiology and Biochemistry 33.2 (2014): 446-456.Read more>>
  • Zeng, Q. J., et al. "Decorin-induced proliferation of avian myoblasts involves the myostatin/Smad signaling pathway." Poultry Science 93.1 (2014): 138-146.Read more>>
  • Liu, Qun, et al. "6-Shogaol induces apoptosis in human leukemia cells through a process involving caspase-mediated cleavage of eIF2α." Molecular cancer 12.1 (2013): 135.Read more>>
VALIDATION IMAGES

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


NIH/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).