bs-0062R [Primary Antibody]
Calretinin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Calretinin

Immunogen Range: 211-271/271


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 794

Swiss Prot: P22676

Source: KLH conjugated synthetic peptide derived from human Calretinin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Calretinin is a calcium-binding protein which is abundant in auditory neurons. It belongs to the calbindin family. Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin1.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100-500)

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Suzui, Masumi, et al. "Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors." Cancer Science 107.7 (2016): 924-935.Read more>>
  • Wang et al. CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. (2018) Cell.Death.Dis. 9:880Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human uterus labeled with Anti-Calretinin/CA Polyclonal Antibody, Unconjugated (bs-0062R) followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Anti-Calretinin/CA Polyclonal Antibody, Unconjugated (bs-0062R) followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum lysates probed with Calretinin Polyclonal Antibody, Unconjugated (bs-0062R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.