Host: Rabbit
Target Protein: Substance P
Immunogen Range: 58-68/129
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6863
Swiss Prot: P20366
Source: KLH conjugated synthetic peptide derived from human SP
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 1_13_
Cross Reactive Species:
Mouse
Rat
Others
Predicted Cross Reactive Species:
Human
Cow
Sheep
Pig
Horse
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
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- Liu, Jun, et al. "Sensory–sympathetic coupling in superior cervical ganglia after myocardial ischemic injury facilitates sympathoexcitatory action via P2X7 receptor." Purinergic Signalling (2013): 1-17.Read more>>
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- Yu, Xi-Jiao, et al. "Expression of substance P in rat periodontal alveolar bone after denervation." International Journal of Clinical and Experimental Pathology 9.2 (2016): 1384-1391.Read more>>
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Formalin-fixed and paraffin embedded rat liver labeled with Rabbit Anti-Substance P Polyclonal Antibody (bs-0065R)at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-Substance P Polyclonal Antibody (bs-0065R) 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat liver tissue labeled with Rabbit Anti-Substance P Polyclonal Antibody(bs-0065R)at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Substance P Polyclonal Antibody, Unconjugated (bs-0065R) at 1:200 overnight at 4°C, DAB staining.
U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Substance P) polyclonal Antibody, Unconjugated (bs-0065R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.