bs-0071R [Primary Antibody]
Neuropeptide Y Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Neuropeptide Y

Immunogen Range: 29-64/97


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4852

Swiss Prot: P01303

Source: KLH conjugated synthetic peptide derived from human NPY

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 11


Cross Reactive Species: Human
Mouse
Rat
Chicken

Predicted Cross Reactive Species: Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Duan, Hubin, et al. "The role of neuropeptide Y and aquaporin 4 in the pathogenesis of intestinal dysfunction caused by traumatic brain injury." Journal of Surgical Research (2013).Read more>>
  • Chen, Shengru, et al. "Changes in nerve fibers and microvessel density in residual spleen after subtotal splenectomy due to portal hypertension." International Journal of Clinical and Experimental Pathology 9.2 (2016): 1557-1567.Read more>>
  • Chen, Guiqian, et al. "The Stimulatory Effect of Cerebral Intraventricular Injection of cNPY on Precocial Feeding Behavior in Neonatal Chicks (Gallus domesticus)." PloS one 11.4 (2016): e0153342.Read more>>
  • Dong et al. Anti-epileptic effects of neuropeptide Y gene transfection into the rat brain. (2013) Neural.Regen.Res. 8:1307-15Read more>>
  • Dong et al. Anti-epileptic effects of neuropeptide Y gene transfection into the rat brain. (2013) Neural.Regen.Res. 8:1307-15Read more>>
  • Quancheng Liu. et al. FumDSB Can Reduce the Toxic Effects of Fumonisin B1 by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs. Toxins. 2021 Dec;13(12):874Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-0071R) 1:400 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Neuropeptide Y Polyclonal Antibody, Unconjugated (bs-0071R) at 1:400 overnight at 4°C, DAB staining.