bs-0078R [Primary Antibody]
TNF alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNF alpha

Immunogen Range: 86-150/233


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7124

Swiss Prot: P01375

Source: KLH conjugated synthetic peptide derived from human TNF alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 17/26


Cross Reactive Species: Human
Mouse
Rat
Pig
Rabbit
Others

Predicted Cross Reactive Species: Dog
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu, Hong-Wu, et al. "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." BMC cancer 13.1 (2013): 247.Read more>>
  • Li, Kang, et al. "CD14 overexpression upregulates TNF-??-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells."Molecular and cellular biochemistry 376.1-2 (2013): 137-143.3Read more>>
  • Liu et al. Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination. (2017) Cell.Death.Diffe. 24:683-693Read more>>
  • Xu et al. Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis. (2016) Mol.Med.Re. 13:4167-74Read more>>
  • Liu et al. Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination. (2017) Cell.Death.Diffe. 24:683-693Read more>>
  • Fang et al. Intrathecal transplantation of bone marrow stromal cells attenuates blood-spinal cord barrier disruption induced by spinal cord ischemia-reperfusion injury in rabbits. (2013) J.Vasc.Sur. 58:1043-52Read more>>
  • Bi et al. miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα. (2016) Mol.Ther.Nucleic.Acid. 5:e368Read more>>
  • Yang et al. Supplement of 5-hydroxytryptophan before induction suppresses inflammation and collagen-induced arthritis. (2015) Arthritis.Res.The. 17:364Read more>>
  • Chen et al. Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels. (2014) Exp.Ther.Me. 8:464-470Read more>>
  • Chen et al. Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels. (2014) Exp.Ther.Me. 8:464-470Read more>>
  • Han et al. Electroacupuncture modulated the inflammatory reaction in MCAO rats via inhibiting the TLR4/NF-κB signaling pathway in microglia. (2015) Int.J.Clin.Exp.Patho. 8:11199-205Read more>>
  • Bai YH et al. LncRNA NEAT1 promotes inflammatory response and induces corneal neovascularization.( 2018) J Mol Endocrinol. Oct 15;61(4):231-239Read more>>
  • Chen L et al. Forsythiaside prevents β-amyloid-induced hippocampal slice injury by upregulating 2-arachidonoylglycerol via cannabinoid receptor 1-dependent NF-κB pathway. Neurochem Int. 2019 May;125:57-66.Read more>>
  • Liang Y et al. Neuroprotective Effect of Echinacoside in Subacute Mouse Model of Parkinson's Disease. Biomed Res Int. 2019 Jan 30;2019:4379639. Read more>>
  • Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63.Read more>>
  • Luo Q et al. Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in PolyphyllinI-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells. Biomed Res Int. 2018 Dec 25;2018:5241941.Read more>>
  • Yang B et al. Polychlorinated Biphenyl Quinone Promotes Macrophage-Derived Foam Cells Formation. Chem Res Toxicol. 2019 Nov 13. Read more>>
  • Jiang S et al. Effect of Illicium verum or Eucommia ulmoides leaf extracts on the anti-stress ability, and mRNA and protein expression of Nrf2 and TNF-α in Duroc × Landrace × Yorkshire and Chinese native Licha-black nursery piglets. J Anim Physiol Anim Nutr (Berl). 2019 Oct 31. Read more>>
  • Yang B et al. Polychlorinated Biphenyl Quinone Promotes Macrophage-Derived Foam Cell Formation. Chem Res Toxicol. 2019 Nov 13.Read more>>
  • Lin J et al. The P2Y14 receptor in the trigeminal ganglion contributes to the maintenance of inflammatory pain. Neurochem Int. 2019 Oct 3;131:104567.Read more>>
  • Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
  • "Murata K et al. Restoring knee joint kinematics after anterior cruciate ligament injury might inhibit synovial membrane inflammation.Sport Sciences for Health. 2018."Read more>>
  • Zheng HX et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020 Apr 15;68(15):4399-4410. Read more>>
  • Ma M et al. Low-dose naltrexone inhibits colorectal cancer progression and promotes apoptosis by increasing M1-type macrophages and activating the Bax/Bcl-2/caspase-3 … Int Immunopharmacol. 2020 Mar 11;83:106388.Read more>>
  • Yang F et al. Copper induces oxidative stress with triggered NF-B pathway leading to inflammatory responses in immune organs of chicken. Ecotoxicol Environ Saf. 2020 Sep 1;200:110715.Read more>>
  • Cheng S et al. Comparative analysis of Longissimus dorsi tissue from two sheep groups identifies differentially expressed genes related to growth, development and meat quality. Genomics. 2020 Sep;112(5):3322-3330.Read more>>
  • Han X et al. [6]\Gingerol Ameliorates ISO\Induced Myocardial Fibrosis by Reducing Oxidative Stress, Inflammation, and Apoptosis through Inhibition of TLR4/MAPKs/NF\B Pathway. Mol Nutr Food Res. 2020 Jul;64(13):e2000003.Read more>>
  • Zhang Q et al. Transcriptome Analysis of the Gene Expression Profiles Associated with Fungal Keratitis in Mice Based on RNA-Seq. Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):32.Read more>>
  • Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
  • Tianyang Huanget al. \Glucan ameliorates nonalcoholic steatohepatitis induced by methionine and choline\deficient diet in mice. J Food Biochem. 2020 Jul 26;e13408.Read more>>
  • Wang C et al. Lysyl oxidase suppresses the inflammatory response in anterior cruciate ligament fibroblasts and promotes tissue regeneration by targeting myotrophin via the nuclear factor-kappa B pathway. J Tissue Eng Regen Med. 2020 Aug;14(8):1063-1076.Read more>>
  • Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28Read more>>
  • Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919Read more>>
  • Siqi Ying. et al. Low-intensity Pulsed Ultrasound regulates alveolar bone homeostasis in experimental Periodontitis by diminishing Oxidative Stress. Theranostics. 2020; 10(21): 9789C9807Read more>>
  • Lei Xianghong. et al. Micheliolide Attenuates Lipopolysaccharide-Induced Inflammation by Modulating the mROS/NF-B/NLRP3 Axis in Renal Tubular Epithelial Cells. Mediat Inflamm. 2020;2020:3934769Read more>>
  • Gaoyang Guo. et al. A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation. Acta Biomater. 2021 Jan;119:89Read more>>
  • Wenshuang Liao. et al. Illicium verum and Eucommia ulmoides leaf extracts promote nutrient availability and antioxidant capacity in piglets by upregulating duodenal and jejunal Nrf2/TNF\. 2021 Mar 26Read more>>
  • Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brainCliver axis in rats. 2021 May 18Read more>>
  • Fangyu Qiao. et al. 4-Octyl itaconate modified demineralized bone matrix scaffold improves bone repair by regulating early inflammation. Chem Eng J. 2021 Dec;425:13149Read more>>
  • Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359Read more>>
  • Liu Z. et al. A Novel Hydrogen Sulfide Donor Reduces Pilocarpine-Induced Status Epilepticus and Regulates Microglial Inflammatory Profile.. Front Cell Neurosci. 2021 Dec;15:780447-780447Read more>>
  • Xuefang Hao. et al. Biomimetic and responsive nanoparticles loading JQ1 for dual-targeting treatment of vascular restenosis via multiple actions. Chem Eng J. 2021 Nov;:133452Read more>>
  • Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-B signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965Read more>>
  • Hongwu Meng. et al. Interleukin-9 attenuates inflammatory response and hepatocyte apoptosis in alcoholic liver injury. Life Sci. 2022 Jan;288:12018Read more>>
  • Haiyan Li. et al. L-arabinose alleviates diabetes-aggravated cerebral ischemic injury by repairing the blood-brain barrier via downregulating NF-B signals. J Funct Foods. 2021 Dec;87:104839Read more>>
  • Yu, Hanjie. et al. Elevation of _-1,3 fucosylation promotes the binding ability of TNFR1 to TNF-_ and contributes to osteoarthritic cartilage destruction and apoptosis. ARTHRITIS RES THER. 2022 Dec;24(1):1-14Read more>>
  • Zhen-Ni Liu. et al. Blockade of the P2Y2 Receptor Attenuates Alcoholic Liver Inflammation by Targeting the EGFR-ERK1/2 Signaling Pathway. DRUG DES DEV THER. Drug Des Dev Ther. 2022 Apr;16:1107-112Read more>>
  • Liu Yang. et al. Protective effect of isopsoralen on UVB-induced injury in HaCaT cells via the ER and p38MAPK signaling pathways. J FOOD BIOCHEM. 2022 Apr 12Read more>>
  • Popova, Velichka. et al. Assessment of TNF-_ expression in unstable atherosclerotic plaques, serum IL-6 and TNF-_ levels in patients with acute coronary syndrome and rheumatoid arthritis. RHEUMATOL INT. 2022 May;:1-8Read more>>
  • Yuge Liao. et al. Inorganic mercury induces liver oxidative stress injury in quails by inhibiting Akt/Nrf2 signal pathway. INORG CHEM COMMUN. 2022 Aug;142:109603Read more>>
VALIDATION IMAGES

Antigen: bs-0078P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450


Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\n


Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\n


Human liver lysates probed with Anti TNF alpha Polyclonal Antibody, Unconjugated (bs-0078R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 17kD, 26kD. Observed band size:26kD.


Lane 1: Human A431 cell lysates; Lane 2: Human A431 cell lysates probed with TNF alpha Polyclonal Antibody, Unconjugated (bs-0078R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.