bs-0079R [Primary Antibody]
CD19 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD19

Immunogen Range: 485-556/556


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12478

Swiss Prot: F1LNH2

Source: KLH conjugated synthetic peptide derived from rat CD19

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 59


Cross Reactive Species: Human
Mouse
Rat
Sheep

Predicted Cross Reactive Species: Cow
Pig
Horse
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Reese, Shannon R., et al. "Calcineurin Inhibitor Minimization With Ixazomib, an Investigational Proteasome Inhibitor, for the Prevention of Antibody Mediated Rejection in a Preclinical Model." Transplantation (2015).Read more>>
  • Takahashi, Yuichi, Yohei Ikezumi, and Akihiko Saitoh. "Rituximab protects podocytes and exerts anti‐proteinuric effects in rat adriamycin‐induced nephropathy independent of B‐lymphocytes." Nephrology (2016).Read more>>
  • Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.Read more>>
  • Goodwin, Justin, et al. "Targeting HIF-1α/PDK1 Axis by Dichloroacetate (DCA) Suppresses Bleomycin-induced Pulmonary Fibrosis." American Journal of Respiratory Cell and Molecular Biology ja (2017).Read more>>
  • Li T et al. Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis.R Soc Open Sci. 2019 Jul 3;6(7):181604. Read more>>
  • Yihan Lu? et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.Read more>>
  • Gitto SB et al. Identification of a novel IL-5 signaling pathway in chronic pancreatitis and crosstalk with pancreatic tumor cells. Cell Commun Signal. 2020 Jun 17;18(1):95.Read more>>
  • Othman Hemn. et al. Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo. Evid-Based Compl Alt. 2020;2020:8764096Read more>>
  • Taotao Li. et al. Integrating miRNA and mRNA Profiling to Assess the Potential miRNACmRNA Modules Linked With Testicular Immune Homeostasis in Sheep. Front Vet Sci. 2021; 8: 647153Read more>>
  • Sylvia Hartmann. et al. Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas. Cancers. 2021 Jan;13(20):5208Read more>>
  • Hong Cheng. et al. Bortezomib alleviates antibody-mediated rejection in kidney transplantation by facilitating Atg5 expression. 2021 Nov 03Read more>>
  • Maoyu Ye. et al. Long-Term Exposure to Sulfur Dioxide Before Sensitization Decreased the Production of Specific IgE in HDM-Sensitized Allergic Rhinitis Mice. J INFLAMM RES. 2022; 15: 2477249Read more>>
  • Jinsheng Li. et al. Micro/nano-topography Promotes Osteogenic Differentiation of Bone Marrow Stem Cells by Regulating Periostin Expression. COLLOID SURFACE B. 2022 Jul;:1127Read more>>
  • Hirona Kugo. et al. Tricaprin can prevent the development of AAA by attenuating aortic degeneration. BIOMED PHARMACOTHER. 2023 Apr;160:114299Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-CD19 Polyclonal Antibody (bs-0079R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded mouse embryo tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD19 Polyclonal Antibody, Unconjugated (bs-0079R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


Human Raji cells probed with CD19 Polyclonal Antibody, Unconjugated (bs-0079R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).