bs-0080R [Primary Antibody]
CD20 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD20

Immunogen Range: 133-182/297


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 931

Swiss Prot: P11836

Source: KLH conjugated synthetic peptide derived from human CD20

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

B lymphocytes marker CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
FCM(1:100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100-500)

Predicted Molecular Weight: 33


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ane M. Andres. et al. Graft infusion of adipose\derived mesenchymal stromal cells to prevent rejection in experimental intestinal transplantation: A feasibility study. 2021 Feb 05Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD20 Polyclonal Antibody (bs-0080R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human larynocarcinoma labeled with Anti-CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Bone lysates probed with CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.