Host: Rabbit
Target Protein: caspase-3 p12 subunit
Immunogen Range: 201-277/277
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 836
Source: KLH conjugated synthetic peptide derived from human caspase-3 p12 subunit
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: Dec-32
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0087R) at 1:500 overnight at 4°C, DAB staining.
Hela cells(black) were fixed with 70% ice-cold methanol overnight at 4℃,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with caspase-3 p12 Antibody(bs-0087R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HL-60 cells(black) were fixed with 70% ice-cold methanol overnight at 4℃,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Antibody(bs-0087R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
NIH/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with caspase-3 p12 subunit Polyclonal Antibody(bs-0087R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HL-60 cell lysates probed with caspase-3 p12 subunit Polyclonal Antibody, Unconjugated (bs-0087R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.