bs-0091R [Primary Antibody]
MUC16 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CA125

Immunogen Range: 250-350/2008


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 94025

Swiss Prot: Q8WXI7

Source: KLH conjugated synthetic peptide derived from human CA125

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
FCM(1:100-500)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100)

Predicted Molecular Weight: 200


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xie, Chao, Chaoqing Dong, and Jicun Ren. "Study on homogeneous competitive immune reaction by fluorescence correlation spectroscopy: Using synthetic peptide as antigen." Talanta 79.3 (2009): 971-974.Read more>>
  • Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415.Read more>>
  • Guo, Yuna, et al. "R-ketorolac Targets Cdc42 and Rac1 and Alters Ovarian Cancer Cell Behaviors Critical for Invasion and Metastasis." Molecular Cancer Therapeutics (2015): molcanther-0419.Read more>>
  • Zong, Chen, et al. "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." Biosensors and Bioelectronics 43 (2013): 372-378.Read more>>
  • Liu et al. Increased autophagy in EOC re-ascites cells can inhibit cell death and promote drug resistance. (2018) Cell.Death.Dis. 9:419Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MUC16 Polyclonal Antibody, Unconjugated (bs-0091R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.