bs-0095R [Primary Antibody]
Calponin 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Calponin 1

Immunogen Range: 201-297/297

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1264

Swiss Prot: P51911

Source: KLH conjugated synthetic peptide derived from human Calponin 1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 33

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Du, Fengyi, et al. "Gradient nanofibrous chitosan/poly ?-caprolactone scaffolds as extracellular microenvironments for vascular tissue engineering." Biomaterials (2011).Read more>>
  • Syedain, Zeeshan, et al. ʺImplantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery.ʺ Tissue Engineering (2014).Read more>>
  • Zhao, Yuechao, et al. "Estrogen-Induced CCN1 is Critical for Establishment of Endometriosis-like Lesions in Mice." Molecular Endocrinology (2014).Read more>>
  • Cai, Jinghua, et al. "Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2." Oncology ReportsRead more>>
  • HAYASHI, TAKUMA, et al. "Characterization of Leiomyomatoid Angiomatous Neuroendocrine Tumour (LANT)-like Tumour in the Myometrium with Histopathological Examination." Anticancer Research 37.4 (2017): 1765-1772.Read more>>
  • Meng et al. Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene. (2017) Oncol.Let. 14:1152-1156Read more>>
  • Han X et al. The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-1/Smad3 pathwayBiomed Pharmacother.2020 Dec 24;134:111135.Read more>>
  • Yinping Zhao. et al. A novel mechanism of inhibiting in-stent restenosis with arsenic trioxide drug-eluting stent: Enhancing contractile phenotype of vascular smooth muscle cells via YAP pathway. Bioact Mater. 2021 Feb;6:375Read more>>
  • Huizhu Zhang. et al. Regulatory effects of HIF-1 and HO-1 in hypoxia-induced proliferation of pulmonary arterial smooth muscle cells in yak. Cell Signal. 2021 Nov;87:11014Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Calponin 1/COLP Polyclonal Antibody, Unconjugated (bs-0095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Calponin 1/COLP Polyclonal Antibody, Unconjugated (bs-0095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with anti-Calponin 1/COLP Polyclonal Antibody (bs-0095R), Unconjugated followed by conjugation to the secondary antibody and DAB staining


H9C2 cells probed with Rabbit Anti-Calponin 1 Polyclonal Antibody (bs-0095R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)


Mouse urinary Bladder lysates probed with Calponin 1 Polyclonal Antibody, Unconjugated (bs-0095R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C