bs-0103R [Primary Antibody]
TGF Beta 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TGF Beta 1

Immunogen Range: 100-150/390


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7040

Swiss Prot: P01137

Source: KLH conjugated synthetic peptide derived from human TGF beta 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fang DP et al. Platelet‐rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF‐β/SMAD signaling pathway. J Cell Physiol. 2019;1–11.Read more>>
  • Wang H et al. The effects of a hirudin/liposome complex on a diabetic nephropathy rat model. BMC Complement Altern Med. 2019 Jun 6;19(1):118.Read more>>
  • Yan Y et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020 Jan 2;25(1). pii: E192. Read more>>
  • Yan J et al. Platelet‐derived microvesicles promote endothelial progenitor cell proliferation in intimal injury by delivering TGF‐β1. FEBS J. 2020 Mar 13Read more>>
  • Qi SS et al. Protective Effects of Chromium Picolinate Against Diabetic-Induced Renal Dysfunction and Renal Fibrosis in Streptozotocin-Induced Diabetic Rats. Biomolecules. 2020 Mar 4;10(3). Read more>>
  • Lina Wuet al. Involvement of miR\27a\3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress. J Cell Physiol. 2021 Feb;236(2):1454-1468.Read more>>
  • Dan Panet al. Morphological Alteration and TGF-1 Expression in Multifidus with Lumbar Disc Herniation. Indian J Orthop. 2020 Jul 29;54(Suppl 1):141-149.Read more>>
  • Yuanbo Cui. et al. HOXD1 functions as a novel tumor suppressor in kidney renal clear cell carcinoma. 2021 Feb 19Read more>>
  • E Xiang. et al. Human umbilical cord-derived mesenchymal stem cells prevent the progression of early diabetic nephropathy through inhibiting inflammation and fibrosis. Stem Cell Res Ther. 2020 Dec;11(1):1-14Read more>>
  • Deng Xinqi. et al. Protective Effects and Metabolic Regulatory Mechanisms of Shenyan Fangshuai Recipe on Chronic Kidney Disease in Rats. Evid-Based Compl Alt. 2020;2020:5603243Read more>>
  • Liu, Tao. et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021 Dec;23(1):1-15Read more>>
  • Anqi Liu. et al. Core fucosylation involvement in the paracrine regulation of proteinuria-induced renal interstitial fibrosis evaluated with the use of a microfluidic chip. Acta Biomater. 2022 FebRead more>>
  • Yingchun Luo. et al. Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis. EBIOMEDICINE. 2022 Aug;82:104087Read more>>
  • Zhe Sui. et al. Ginsenoside Rg3 has effects comparable to those of ginsenoside re on diabetic kidney disease prevention in db/db mice by regulating inflammation, fibrosis and PPAR. MOL MED REP. 2023 Apr;27(4):1-1Read more>>
  • Fadian Ding. et al. Adropin attenuates pancreatitis?associated lung injury through PPAR phosphorylation?related macrophage polarization. INT J MOL MED. 2023 Oct;52(4):1-1Read more>>
  • Xuejie Han. et al. Evolocumab prevents atrial fibrillation in rheumatoid arthritis rats through restraint of PCSK9 induced atrial remodeling. J ADV RES. 2023 SepRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human pituitary tumor labeled with Anti-TGF-beta1 Polyclonal Antibody (bs-0103R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


K562 Cell lysates probed with TGF Beta 1 Polyclonal Antibody, unconjugated (bs-0103R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. Bottom band is the Latency-associated peptide (LAP).


Lane 1: Human K562 cell lysates; Lane 2: Human U-2OS cell lysates probed with TGF Beta 1 Polyclonal Antibody, Unconjugated (bs-0103R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.