bs-0107R
[Primary Antibody]
beta Amyloid 1-42 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: beta Amyloid 1-42
Immunogen Range: 1-42/42
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 351
Swiss Prot: P05067
Source: KLH conjugated synthetic peptide of human beta-Amyloid(1-42)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. beta Amyloid and peptides have been developed as tools for elucidating the biology of Alzheimer's disease.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 4.4
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang, Qinan, et al. "Transient Focal Cerebral Ischemia/Reperfusion Induces Early and Chronic Axonal Changes in Rats: Its Importance for the Risk of Alzheimer's Disease." PloS one 7.3 (2012): e33722.Read more>>
- Lucassen, Paul J. "The origin and development of plaques and phosphorylated tau are associated with axonopathy in Alzheimer’s disease." Neuroscience bulletin 27.5 (2011): 287-299.Read more>>
- Tian, Yue, et al. "Minocycline Alleviates Sevoflurane-Induced Cognitive Impairment in Aged Rats." Cellular and Molecular Neurobiology (2015): 1-10.Read more>>
- Wang, Chengke, and Zhenxin Wang. "Studying the relationship between cell cycle and Alzheimer’s disease by gold nanoparticle probes." Analytical Biochemistry (2015).Read more>>
- Li, Yu, et al. "Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway."Journal of Molecular Neuroscience (2015): 1-11.Read more>>
- Behairi, Nassima, et al. "All-trans retinoic acid (ATRA) prevents lipopolysaccharide-induced neuroinflammation, amyloidogenesis and memory impairment in aged rats." Journal of Neuroimmunology (2016).Read more>>
- Kang, Lin, et al. "Dihydrotestosterone treatment delays the conversion from mild cognitive impairment to Alzheimer's disease in SAMP8 mice."Hormones and behavior 65.5 (2014): 505-515.Read more>>
- Vinothkumar, G., et al. "Abnormal Amyloid β 42 expression and increased oxidative stress in plasma of CKD patients with cognitive dysfunction: A small scale case control study comparison with Alzheimer's disease." BBA Clinical (2017).Read more>>
- Chen L et al. Methamphetamine exposure induces neuropathic protein β-Amyloid expression.(2018) Toxicol In Vitro.Read more>>
- Chen YC et al. Indole Compound NC009-1 Augments APOE and TRKA in Alzheimer's Disease Cell and Mouse Models for Neuroprotection and Cognitive Improvement. J Alzheimers Dis. 2019;67(2):737-756.Read more>>
- Jiang X et al. Icariin Ameliorates Amyloid Pathologies by Maintaining Homeostasis of Autophagic Systems in Aβ 1–42-Injected Rats. Neurochemical Research. 2019. Read more>>
- Honghe Xiao et al. Shenzao jiannao oral liquid, an herbal formula, ameliorates cognitive impairments by rescuing neuronal death and triggering endogenous neurogenesis in AD-like mice induced by a combination of A42 and scopolamine. J Ethnopharmacol. 2020 Sep 15;259:112957.Read more>>
- Kai Kanget al. FGF21 attenuates neurodegeneration through modulating neuroinflammation and oxidant-stress. Biomed Pharmacother. 2020 Sep;129:110439.Read more>>
- Chunyue Wang. et al. Isoforsythiaside Attenuates Alzheimers Disease via Regulating Mitochondrial Function Through the PI3K/AKT Pathway. Int J Mol Sci. 2020 Jan;21(16):5687Read more>>
- Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimers disease via the relief of endoplasmic reticulum stress in A-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16Read more>>
- Jian-Zhong Li. et al. An enriched environment delays the progression from mild cognitive impairment to Alzheimer's disease in senescence?accelerated mouse prone?8 mice. Exp Ther Med. 2021 Nov;22(5):1-12Read more>>
- Pengfei Fu. et al. An integrative analysis of miRNA and mRNA expression in the brains of Alzheimer's disease transgenic mice after real-world PM2.5 exposure. J Environ Sci-China. 2022 Dec;122:25Read more>>
- Lingling Dong. et al. Anti-inflammatory effect of Rhein on ulcerative colitis via inhibiting PI3K/Akt/mTOR signaling pathway and regulating gut microbiota. 2022 Mar 01Read more>>
- Shixin Ding. et al. Chronic glucocorticoid exposure accelerates A generation and neurotoxicity by activating calcium-mediated CN-NFAT1 signaling in hippocampal neurons in APP/PS1 mice. FOOD CHEM TOXICOL. 2022 Oct;168:113407Read more>>
- Xue-Lian Meng. et al. Total alkaloids from the seed embryo of Nelumbo nucifera Gaertn. improve cognitive impairment in APP/PS1 mice and protect A-damaged PC12 cells. NUTR NEUROSCI. 2022 Nov 12Read more>>
- Jing-Su Xue. et al. Dauricine alleviates cognitive impairment in Alzheimer's disease mice induced by D-galactose and AlCl3 via the Ca2+/CaM pathway. TOXICOL APPL PHARM. 2023 Jul;:116613Read more>>
- Yue Guan. et al. Hericium coralloides Ameliorates Alzheimers Disease Pathologies and Cognitive Disorders by Activating Nrf2 Signaling and Regulating Gut Microbiota. NUTRIENTS. 2023 Jan;15(17):3799Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-beta-Amyloid(1-42) Polyclonal Antibody (bs-0107R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U87MG cell lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human U251 cell lysates probed with beta-Amyloid 1-40 (CT) Polyclonal Antibody, Unconjugated (bs-0107R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.