bs-0112R-HRP [Conjugated Primary Antibody]
APP/Amyloid Precursor Protein Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Amyloid Precursor Protein

Immunogen Range: 381-480/770

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 351

Swiss Prot: P05067

Source: KLH conjugated synthetic peptide derived from human of human APP

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin.

Storage Condition: Store at 4°C for 12 months.

Background: This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Potter AS, Potter SS (2015) Molecular Anatomy of Palate Development. PLoS ONE 10(7): e0132662. doi:10.1371Read more>>
  • Wang, Chengke, and Zhenxin Wang. "Studying the relationship between cell cycle and Alzheimer’s disease by gold nanoparticle probes." Analytical Biochemistry (2015).Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-APP/Amyloid Precursor Protein Polyclonal Antibody (bs-0112R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with APP Polyclonal Antibody, Unconjugated (bs-0112R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: A431 lysates; Lane 2: Raji lysates; Jurkat lysates probed with Amyloid Precursor Protein Antibody, Unconjugated (bs-0112R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Rat eye lysates probed with Amyloid Precursor Protein Antibody, Unconjugated (bs-0112R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.