bs-0118R [Primary Antibody]
Androgen receptor Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Androgen receptor

Immunogen Range: 825-919/919


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 367

Swiss Prot: P10275

Source: KLH conjugated synthetic peptide derived from human AR

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 43/101


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Madka, Venkateshwar, et al. "Targeting mTOR and p53 signaling inhibits muscle invasive bladder cancer in vivo." Cancer Prevention Research 9.1 (2016): 53-62.Read more>>
  • Liu et al. Bak Foong pills combined with metformin in the treatment of a polycystic ovarian syndrome rat model. (2015) Oncol.Let. 10:1819-1825Read more>>
  • Ma et al. miR-762 promotes porcine immature Sertoli cell growth via the ring finger protein 4 (RNF4) gene. (2016) Sci.Re. 6:32783Read more>>
  • Zhao Z et al. LncNONO-AS regulates AR expression by mediating NONO. Theriogenology. 2019 Nov 7. pii: S0093-691X(19)30479-0. Read more>>
  • Yang X et al. AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR\124aReprod Domest Anim.2020 Dec 10.Read more>>
  • Longfei Xiao. et al. Dihydrotestosterone regulation of cyclooxygenase-2 expression in bovine endometrial epithelium cells by androgen receptor mediated EGFR/PI3K/Akt pathway. J Steroid Biochem. 2021 Nov;214:106001Read more>>
  • Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
  • Chuanli Zhang. et al. Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes. Exp Eye Res. 2022 Feb;:108983Read more>>
  • Yixuan Liang. et al. Dihydrotestosterone mediates the inflammation effect under lipopolysaccharides in bovine endometrial epithelial cells via AR blockading TLR4/MyD88 signaling pathway. ANIM REPROD SCI. 2023 Aug;255:107292Read more>>
  • Huang Jing. et al. Effects of exposure to PM2.5 during pregnancy on the multigenerational reproductive outcomes of male mouse offspring and the role of Sertoli cells. ENVIRON SCI POLLUT R. 2023 Sep;:1-13Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Androgen Receptor Polyclonal Antibody, Unconjugated (bs-0118R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.