DATASHEET
Host:
Rabbit
Target Protein:
Androgen receptor
Immunogen Range:
825-919/919
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
367
Swiss Prot:
P10275
Source:
KLH conjugated synthetic peptide derived from human AR
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
PRODUCT SPECIFIC PUBLICATIONS
- Madka, Venkateshwar, et al. "Targeting mTOR and p53 signaling inhibits muscle invasive bladder cancer in vivo." Cancer Prevention Research 9.1 (2016): 53-62.Read more>>
- Liu et al. Bak Foong pills combined with metformin in the treatment of a polycystic ovarian syndrome rat model. (2015) Oncol.Let. 10:1819-1825Read more>>
- Ma et al. miR-762 promotes porcine immature Sertoli cell growth via the ring finger protein 4 (RNF4) gene. (2016) Sci.Re. 6:32783Read more>>
- Zhao Z et al. LncNONO-AS regulates AR expression by mediating NONO. Theriogenology. 2019 Nov 7. pii: S0093-691X(19)30479-0. Read more>>
- Yang X et al. AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR\124aReprod Domest Anim.2020 Dec 10.Read more>>
- Longfei Xiao. et al. Dihydrotestosterone regulation of cyclooxygenase-2 expression in bovine endometrial epithelium cells by androgen receptor mediated EGFR/PI3K/Akt pathway. J Steroid Biochem. 2021 Nov;214:106001Read more>>
- Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
- Chuanli Zhang. et al. Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes. Exp Eye Res. 2022 Feb;:108983Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Androgen Receptor Polyclonal Antibody, Unconjugated (bs-0118R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Mouse kidney lysates probed with Androgen receptor Polyclonal Antibody, Unconjugated (bs-0118R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.