bs-0126R
[Primary Antibody]
HSP70 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: HSP70
Immunogen Range: 481-534/641
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3303
Swiss Prot: P08107
Source: KLH conjugated synthetic peptide derived from human HSP70
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
In cooperation with other chaperones, Hsp7s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Others
Predicted Cross Reactive Species:
Dog
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Fu‐Xin, et al. "Proteomic analysis of the sea‐island cotton roots infected by wilt pathogen Verticillium dahliae." Proteomics 11.22 (2011): 4296-4309.Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Shao,et al.Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken.(2018) Environmental Science and Pollution Research. 25:7719-7729.Read more>>
- Xu,et al.Ultrasensitive electrochemical sensing of Hg2+ based on thymine-Hg2+-thymine interaction and signal amplification of alkaline phosphatase catalyzed silver deposition.(2018) Biosensors & Bioelectronics. 104:95-101.Read more>>
- Shen H et al. Effects of elevated ambient temperature and local testicular heating on the expressions of heat shock protein 70 and androgen receptor in boar testes. Acta Histochem. 2019 Feb 2. pii: S0065-1281(18)30339-8. Read more>>
- Yang X et al. The Activation of Heat-Shock Protein After Copper (II) and/or Arsenic (III)-Induced Imbalance of Homeostasis, Inflammatory Response in Chicken Rectum. Biol Trace Elem Res. 2019 Aug 31.Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- El-Lakany MA et al. Ovariectomy provokes inflammatory and cardiovascular effects of endotoxemia in rats: Dissimilar benefits of hormonal supplements. Toxicol Appl Pharmacol. 2020 Feb 21;393:114928.Read more>>
- Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282Read more>>
- Shi Wentao. et al. Periodic Heat Stress Licenses EMSC Differentiation into Osteoblasts via YAP Signaling Pathway Activation. Stem Cells Int. 2022;2022:3715471Read more>>
- Ali Tugrul Akin. et al. Chloroquine attenuates chronic hypoxia-induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model. CLIN EXP PHARMACOL P. 2022 May 17Read more>>
- Zihui Wang. et al. Heat stress and hypoxia inhibit the secretion of androgens and induce epithelial-to-mesenchymal transition associated with activated TGF-_/Smad signaling in canine cryptorchidism. REPROD DOMEST ANIM. 2022 Jun 09Read more>>
- Song-Hsien Lin. et al. Effects of Cancer Cell-Derived Nanovesicle Vaccines Produced by the Oxidative Stress-Induced Expression of DAMP and Spontaneous Release/Filter Extrusion in the Interplay of Cancer Cells and Macrophages. BIOMEDICINES. 2022 Aug;10(8):1977Read more>>
- Yue Sun. et al. A single-beam of light priming the immune responses and boosting cancer photoimmunotherapy. J CONTROL RELEASE. 2022 Oct;350:734Read more>>
- Rui Wang. et al. A quantitative proteomic analyses of primary myocardial cell injury induced by heat stress in chicken embryo. J THERM BIOL. 2023 Feb;112:103461Read more>>
- Ozge Goktepe. et al. The effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats. FOOD CHEM TOXICOL. 2023 Jul;177:113816Read more>>
- Karaman Enes. et al. Protective Effects of Boric Acid Taken in Different Ways on Experimental Ovarian ?schemia and Reperfusion. BIOL TRACE ELEM RES. 2023 Sep;:1-14Read more>>
VALIDATION IMAGES
"Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-HSP-70 Polyclonal Antibody (bs-0126R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining\n"
Generously provided by Merlin Levine from National Institutes of Health as part of the Bioss Discovery Program. Mouse brain labeled with Anti-HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:100 overnight. The secondary antibody was AlexaFluor 555 goat anti mouse IgG1 used at 1:250 for one hour.
Mouse bladder lysates probed with HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0126R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.