bs-0127M
[Primary Antibody]
Bax Polyclonal Antibody
DATASHEET
Host: Mouse
Target Protein: Bax
Immunogen Range: 84-175/192
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 581
Swiss Prot: Q07812
Source: KLH conjugated synthetic peptide derived from human Bax
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 21
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Huang, Chuen-Lin, et al. "Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability." Molecular Neurobiology (2015): 1-20.Read more>>
- Sukohar, Asep, and Mohammad Kanedi. "Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and P53, in T47D Breast Cancer Cells." Biomedical and Pharmacology Journal 10.2 (2017): 641-649.Read more>>
- Ma et al. A potential adjuvant chemotherapeutics, 18β-glycyrrhetinic acid, inhibits renal tubular epithelial cells apoptosis via enhancing BMP-7 epigenetically through targeting HDAC2. (2016) Sci.Re. 6:25396Read more>>
- Li et al. A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs. (2017) PLoS.On. 12:e0175407Read more>>
- Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458Read more>>
- Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
- Su C et al. Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats. Mol Med Rep. 2019 Sep 11. Read more>>
- Zhan C et al. Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Toxicol Lett. 2019 Oct 1. pii: S0378-4274(19)30295-4. Read more>>
- Ji X et al. Double-component diazeniumdiolate derivatives as anti-cancer agents. Bioorg Med Chem. 2020 Apr 15;28(8):115405.Read more>>
- Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14Read more>>
- Xuemei Shen. et al. CircINSR Regulates Fetal Bovine Muscle and Fat Development. Front Cell Dev Biol. 2020; 8: 615638Read more>>
- Dan Liu. et al. Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency. Mol Reprod Dev. 2020 Aug;87(8):887-898Read more>>
- Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625Read more>>
- EnShuang Xu. et al. Establishment and transcriptome characterization of tamoxifen-resistant canine mammary gland tumor cells. Res Vet Sci. 2022 FebRead more>>
- Yuelei Zhao. et al. Nonivamide induces brown fat-like characteristics in porcine subcutaneous adipocytes. BIOCHEM BIOPH RES CO. 2022 Sep;619:68Read more>>
- Fang Dai. et al. Effects of long term diabetogenic high fat diet on bone in ovariectomized female rats. BIOTECH HISTOCHEM. 2022 Jun 28Read more>>
- Mikyung Kang. et al. T-cell membrane coating for improving polymeric nanoparticle-based cancer therapy. J IND ENG CHEM. 2022 NovRead more>>
- Liu, Haiping. et al. The oxidative damage induced by lead sulfide nanoparticles in rat kidney. MOL CELL TOXICOL. 2022 Nov;:1-12Read more>>
- Abulaiti Abulizi. et al. Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3 pathway. BIOMED PHARMACOTHER. 2023 Apr;160:11433Read more>>
- Dongmei Jiang. et al. Ferritin heavy chain participated in ameliorating 3-nitropropionic acid-induced oxidative stress and apoptosis of goose follicular granulosa cells. POULTRY SCIENCE. 2023 Feb;:102606Read more>>
- Wen Jiang. et al. Hirsutine ameliorates myocardial ischemia-reperfusion injury through improving mitochondrial function via CaMKII pathway. CLIN EXP HYPERTENS. 2023;45(1):Article: 2192444Read more>>
- Jing Zhang. et al. Effects of dietary folic acid supplementation on lactation performance and mammary epithelial cell development of dairy cows and its regulatory mechanism. ANIM BIOTECHNOL. 2023 Jul 06Read more>>
- Zhang Zhichun. et al. A novel SLC25A1 inhibitor, parthenolide, suppresses the growth and stemness of liver cancer stem cells with metabolic vulnerability. CELL DEATH DISCOV. 2023 Sep;9(1):1-12Read more>>
- Jing Zhang. et al. Dietary supplementation of sodium butyrate enhances lactation performance by promoting nutrient digestion and mammary gland development in dairy cows. ANIM NUTR. 2023 SepRead more>>
- Zheng Meng. et al. Targeted ablation of the left middle cervical ganglion prevents ventricular arrhythmias and cardiac injury induced by AMI. BASIC RES CARDIOL. 2023 Dec;:1-18Read more>>
- Yapeng Liu. et al. Effects of nanoselenium supplementation on lactation performance, nutrient digestion and mammary gland development in dairy cows. ANIM BIOTECHNOL. 2023 Dec 12Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti- Bax Polyclonal Antibody, Unconjugated (bs-0127M) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
L1 mouse cerebrum lysates, probed: Anti-Bax (bs-0127M) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:2000 90min in RT. Predicted and observed band size: 21kD
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: 1: Recombinant human Bax Protein (full length) Overexpression E.coli Lysate lysates probed with Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.