bs-0128R
[Primary Antibody]
PI3 kinase p85 alpha subunit Antibody
DATASHEET
Host: Rabbit
Target Protein: PI3 kinase p85 alpha subunit
Immunogen Range: 501-600/724
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5295
Swiss Prot: P27986
Source: KLH conjugated synthetic peptide derived from human PI3 kinase p85 subunit alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 80
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Xu, Hong-Wu, et al. "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." BMC cancer 13.1 (2013): 247.Read more>>
- Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
- Xu, C., et al. "Proteomics Analysis of Hepatocyte Proliferation Regulated by FGF, PDGF, Insulin, Oncostatin M and Interleukin 2 Signaling Pathways during Rat Liver Regeneration." J Proteomics Computational Biol 1.1 (2014): 8.Read more>>
- An, Xinjiang, et al. "Role of the PTEN/PI3K/VEGF pathway in the development of Kawasaki disease." Experimental and therapeutic medicine11.4 (2016): 1318-1322.Read more>>
- Tian, L-Y., et al. "Anti-diabetic effect of methylswertianin and bellidifolin from Swertia punicea Hemsl. and its potential mechanism." Phytomedicine 17.7 (2010): 533-539.Read more>>
- Bai, Xue, et al. "Effects of water extract and crude polysaccharides from Liriope spicata var. prolifera on InsR/IRS-1/PI3K pathway and glucose metabolism in mice." Journal of ethnopharmacology 125.3 (2009): 482-486.Read more>>
- Wang, Fan, et al. "Defective insulin signaling and the protective effects of dimethyldiguanide during follicular development in the ovaries of polycystic ovary syndrome." Molecular Medicine Reports (2017).Read more>>
- Wang, Fan, et al. "Defective insulin signaling and the protective effects of dimethyldiguanide during follicular development in the ovaries of polycystic ovary syndrome." Molecular Medicine Reports (2017).Read more>>
- Yang et al. Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. (2014) Exp.Ther.Me. 8:563-566Read more>>
- Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
- Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
- Jiang A et al. Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier. Int Immunopharmacol. 2019 Nov 8:105972.Read more>>
- Zhu J et al. SPARC Promotes Self‐Renewal of Limbal Epithelial Stem Cells and Ocular Surface Restoration through JNK and p38‐MAPK Signaling Pathways. Stem Cells. 2019 Oct 23.Read more>>
- Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. MedChemComm. 2018.Read more>>
- Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
- Chen Y et al. Epothilone B prevents lipopolysaccharide-induced inflammatory osteolysis through suppressing osteoclastogenesis via STAT3 signaling pathway. Aging (Albany NY). 2020 Jun 11;12(12):11698-11716.Read more>>
- Fan Q et al. Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement. Evid Based Complement Alternat Med. 2020 Jun 9;2020:2957094.Read more>>
- Cui Z. et al. High Expression of miR-204 in Chicken Atrophic Ovaries Promotes Granulosa Cell Apoptosis and Inhibits Autophagy.. Front Cell Dev Biol. 2020 Nov;8:580072-580072Read more>>
- Han Chen. et al. ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-B signaling pathway. Bmc Cancer. 2020 Dec;20(1):1-1Read more>>
- Xiaolan Wang. et al. The total flavonoids from Selaginella tamariscina (beauv.) Spring improve glucose and lipid metabolism in db/db mice. Iran J Basic Med Sci. 2020 Oct; 23(10): 1286C1292Read more>>
- Jin Dai. et al. NK6 Homeobox 2 Regulated Gastrokin-2 Suppresses Gastric Cancer Cell Proliferation and Invasion via Akt Signaling Pathway. Cell Biochem Biophys. 2021 Mar;79(1):123-131Read more>>
- Yi Chen. et al. The essential oil from the raw and vinegar processed Rhizoma Curcumae ameliorate CCl4-incuded liver fibrosis: integrating network pharmacology and molecular mechanism evaluation. 2021 Mar 17Read more>>
- Jing Chen. et al. Apatinib enhances the anti-tumor effect of paclitaxel via the PI3K/p65/Bcl-xl pathway in triple-negative breast cancer. Ann Transl Med. 2021 Jun; 9(12): 1001Read more>>
- Deng Yang-lin. et al. Prescription of Sageretia hamosa Brongn Relieved Goiter through Promoted Apoptosis of Thyroid Cells via miR-511-3p and PTEN/PI3K/Akt Pathway. J Healthc Eng. 2021;2021:3506559Read more>>
- Mingfa Ling. et al. VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway. Int J Mol Sci. 2021 Jan;22(24):13352Read more>>
- Shanyu Fang. et al. Lanthanum Chloride Sensitizes Cisplatin Resistance of Ovarian Cancer Cells via PI3K/Akt Pathway. Front Med-Lausanne. 2021; 8: 776876Read more>>
- Xinping Song. et al. Lactobacillus plantarum DP189 prevents cognitive dysfunction in D-galactose/AlCl3 induced mouse model of Alzheimers disease via modulating gut microbiota and PI3K/Akt/GSK-3 signaling pathway. 2021 Nov 1Read more>>
- Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476Read more>>
- Ling Junjun. et al. Exploration of Potential Targets and Mechanisms of Fisetin in the Treatment of Non-Small-Cell Lung Carcinoma via Network Pharmacology and In Vitro Validation. EVID-BASED COMPL ALT. 2022;2022:2383527Read more>>
- Huang, Wenjie. et al. The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters. CHIN MED-UK. 2022 Dec;17(1):1-18Read more>>
- endong Yang. et al. Naringenin Ameliorates Hyperuricemia by Regulating Renal Uric Acid Excretion via the PI3K/AKT Signaling Pathway and Renal Inflammation through the NF-B Signaling Pathway. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
- Hong Kyu Lee. et al. TGF-2 antisense oligonucleotide enhances T-cell mediated anti-tumor activities by IL-2 via attenuation of fibrotic reaction in a humanized mouse model of pancreatic ductal adenocarcinoma. BIOMED PHARMACOTHER. 2023 Mar;159:114212Read more>>
- Fan Zhiqin. et al. Phosphorylation of AKT by lysyl oxidase-like 2 activates the PI3K/AKT signaling pathway to promote proliferation, invasion and metastasis in esophageal squamous carcinoma. CLIN TRANSL ONCOL. 2023 Mar;:1-12Read more>>
- Li Li. et al. Regulation of TREM2 on BV2 inflammation through PI3K/AKT/mTOR pathway. BIOTECHNOL GENET ENG. 2023 Apr 26Read more>>
- Qiao Wanchen. et al. Liensinine ameliorates ischemiaCreperfusion-induced brain injury by inhibiting autophagy via PI3K/AKT signaling. FUNCT INTEGR GENOMIC. 2023 Jun;23(2):1-9Read more>>
- Shumin Bi. et al. miR-210 promotes hepatocellular carcinoma progression by modulating macrophage autophagy through PI3K/AKT/mTOR signaling. BIOCHEM BIOPH RES CO. 2023 ApRead more>>
- Hongjuan He. et al. Lycopene alleviates food allergy by modulating the PI3K/AKT pathway in peanut-sensitized BALB/c mice. IMMUNOBIOLOGY. 2023 Aug;:152731Read more>>
- Xiaoxiao Zhang. et al. Tanshinone A participates in the treatment of endometriosis by regulating adhesion, invasion, angiogenesis and inhibition of PI3K/Akt/mTOR signaling pathway. MOL MED REP. 2023 Nov;28(5):1-13Read more>>
- Shanshan Wu. et al. Characterization of lncRNA and mRNA profiles in ciliary body in experimental myopia. EXP EYE RES. 2024 Apr;241:109849Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
H9C2 cells probed with Rabbit Anti-PI3 kinase p85 alpha subunit Polyclonal Antibody (bs-0128R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)
Rat brain lysates probed with Anti PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 80kD. Observed band size: 85kD.
HepG2 cells were stained with PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated(bs-0128R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PI 3 Kinase p85 alpha) polyclonal Antibody, Unconjugated (bs-0128R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.