bs-0152R [Primary Antibody]
Prealbumin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Prealbumin

Immunogen Range: 51-147/147


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 22139

Swiss Prot: P07309

Source: KLH conjugated synthetic peptide derived from mouse Transthyretin/Prealbumin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 14


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Qian, et al. "Proteomic Study on Usnic Acid-induced Hepatotoxicity in Rats." Journal of agricultural and food chemistry (2012).Read more>>
  • Herrick-Davis, Katharine, et al. "Native Serotonin 5-HT2C Receptors are Expressed as Homodimers on the Apical Surface of Choroid Plexus Epithelial Cells." Molecular Pharmacology (2015): mol-114.Read more>>
  • Dai, Keqiang, et al. "Induction of Functional Hepatocyte-Like Cells by Overexpression of FOXA3 and HNF4α in Rat Bone Marrow Mesenchymal Stem Cells." Cells Tissues Organs (2015).Read more>>
  • Ding, Yi, et al. "Overexpression of transcription factor Foxa2 and Hnf1α induced rat bone mesenchymal stem cells into hepatocytes."Cytotechnology (2016): 1-11.Read more>>
  • Wang Z et al. Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persisted in Adult-aged OffspringEndocrinology.2020 Dec 1;161(12):bqaa164.Read more>>
  • Dong X et al. PM2.5 disrupts thyroid hormone homeostasis through activation of the hypothalamic-pituitary-thyroid (HPT) axis and induction of hepatic transthyretin in female rats. Ecotoxicol Environ Saf 2021 Jan 15;208:111720.Read more>>
  • Zhou, Can-Can. et al. Lead Exposure in Developmental Ages Promotes A Accumulation by Disturbing A Transportation in Blood-Cerebrospinal Fluid Barrier/BloodCBrain Barriers and Impairing A Clearance in the Liver. 2021 Nov 17Read more>>
  • Dong, Xinwen. et al. Protective effects of curcumin against thyroid hormone imbalance after gas explosion-induced traumatic brain injury via activation of the hypothalamic-pituitary-thyroid axis in male rats. ENVIRON SCI POLLUT R. 2022 May;:1-13Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TTR Prealbumin beta Polyclonal Antibody, Unconjugated (bs-0152R) at 1:400 overnight at 4°C, DAB staining.


HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Prealbumin Antibody(bs-0152R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).