bs-0154R [Primary Antibody]
GAP43 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GAP43

Immunogen Range: 9-100/238


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14432

Swiss Prot: P06837

Source: KLH conjugated synthetic peptide derived from mouse GAP43

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 25/46


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jiang et al. Effect of Frankincense Extract on Nerve Recovery in the Rat Sciatic Nerve Damage Model. (2016) Evid.Based.Complement.Alternat.Med. 2016:3617216Read more>>
  • Zhai X et al. L-lactate preconditioning promotes plasticity-related proteins expression and reduces neurological deficits by potentiating GPR81 signaling in rat traumatic brain injury model. Brain Res. 2020 Nov 1;1746:146945.Read more>>
  • Sheng Cao. et al. Ultrasound-targeted microbubble destruction-mediated Ang1 gene transfection improves left ventricular structural and sympathetic nerve remodeling in canines with myocardial infarction. Ann Transl Med. 2021 Feb; 9(3): 221Read more>>
  • Han Yang. et al. A Novel Targeted and High\Efficiency Nanosystem for Combinational Therapy for Alzheimer's Disease. Adv Sci. 2020 Oct;7(19):1902906Read more>>
  • Yifan Wang. et al. Positive effect of Astragaloside IV on neurite outgrowth via talin\dependent integrin signaling and microfilament force. J Cell Physiol. 2021 Mar;236(3):2156-2168Read more>>
  • Pingan Chen. et al. The influence of inhibiting renal neural regeneration on the efficacy of renal denervation to chronic heart failure. 2021 Oct 22Read more>>
  • Erdal Ayhan I_ik. et al. Use of Erythropoietin and Fibrin Glue Mixture for Peripheral Nerve Repair. Plast Reconstr Surg. 2022 Feb;149(2):395-403Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-GAP-43 Polyclonal Antibody, Unconjugated (bs-0154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-GAP-43 Polyclonal Antibody, Unconjugated (bs-0154R) at 1:300 followed by conjugation to the secondary antibody and DAB staining


HeLa cells probed with GAP43 Polyclonal Antibody, unconjugated (bs-0154R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)