bs-0155R [Primary Antibody]
CRP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CRP

Immunogen Range: 125-224/224


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1401

Swiss Prot: P02741

Source: KLH conjugated synthetic peptide derived from human CRP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Mouse
Rabbit

Predicted Cross Reactive Species: Rat
Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Guo, Fang, et al. "Fibrinogen, fibrin, and FDP induce C-reactive protein generation in rat vascular smooth muscle cells: Pro-inflammatory effect on atherosclerosis." Biochemical and biophysical research communications 390.3 (2009): 942-946.Read more>>
  • Ding, Lin, et al. "Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolism." Journal of Functional Foods 32 (2017): 90-97.Read more>>
  • Zhang L et al. Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice. Food & Function. 2019  Read more>>
  • Rong Z et al. A C-reactive protein immunosensor based on platinum nanowire/titania nanotube composite sensitized electrochemiluminescence. Talanta, 2019.205, 120135. Read more>>
  • Liu X et al. Polydopamine-based molecular imprinted optic microfiber sensor enhanced by template-mediated molecular rearrangement for ultra-sensitive C-reactive protein detection. Chemical Engineering Journal. 2020, May 387: 124074.Read more>>
  • Jin Z et al. Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway. Biomed Pharmacother. 2020 May;125:110015. Read more>>
  • Xiaoxuan Lei. et al. Human Salivary Histatin-1 Is More Efficacious in Promoting Acute Skin Wound Healing Than Acellular Dermal Matrix Paste. Front Bioeng Biotech. 2020; 8: 999Read more>>
  • Yu Fu. et al. C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms. FRONT IMMUNOL. 2023; 14: 1233807Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:400 overnight at 4°C, DAB staining.