bs-0158R [Primary Antibody]
Neurotrophin 4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Neurotrophin 4

Immunogen Range: 121-210/210


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4909

Swiss Prot: P34130

Source: KLH conjugated synthetic peptide derived from human NT-4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Neurotrophin 4 (NT4; synonymous with NT5) belongs to the NGF beta family and is a survival factor for peripheral sensory sympathetic neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. NT4 levels are highest in the prostate, with lower levels in thymus, placenta, and skeletal muscle. NT4 is also expressed in embryonic and adult tissues.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 14/27


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Neurotrophin 4/NT-4/NT-5 Polyclonal Antibody, Unconjugated (bs-0158R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 mouse embryo lysates L2 mouse brain lysates probed with Anti Neurotrophin 4/NT-4/NT-5 Polyclonal Antibody, Unconjugated (bs-0158R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 14/27kDa. Observed band size:27kD.