bs-0161R [Primary Antibody]
p75 NGF Receptor Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: p75 NGF Receptor

Immunogen Range: 301-400/427


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24596

Swiss Prot: P07174

Source: KLH conjugated synthetic peptide derived from rat NGFR

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake. Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Paczkowska, Edyta, et al. ʺHumoral Activity of Cord Blood-Derived Stem/Progenitor Cells: Implications for Stem Cell-Based Adjuvant Therapy of Neurodegenerative Disorders.ʺ PloS one 8.12 (2013): e83833.Read more>>
  • Shan, Tengfei, et al. "Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β‐catenin signaling." Cell Biology International (2014).Read more>>
  • Zhang, Ying, et al. "Exendin-4 promotes proliferation of adipose-derived stem cells through ERK and JNK signaling pathways." In Vitro Cellular & Developmental Biology-Animal (2016): 1-9.Read more>>
  • Śmieszek, Agnieszka, et al. "Antioxidant and Anti-Senescence Effect of Metformin on Mouse Olfactory Ensheathing Cells (mOECs) May Be Associated with Increased Brain-Derived Neurotrophic Factor Levels—An Ex Vivo Study." International Journal of Molecular Sciences 18.4 (2017): 872.Read more>>
  • Zhang K et al. Multipotent stem cells with neural crest stem cells characteristics exist in bovine adipose tissue. Biochem Biophys Res Commun. 2019 Nov 29.Read more>>
  • Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
  • Yoshito Mizoguchi. et al. ProBDNF induces sustained elevation of intracellular Ca2+ possibly mediated by TRPM7 channels in rodent microglial cells. 2021 Mar 19Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated (bs-0161R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse brain lysates probed with Anti-p75 NGF Receptor Polyclonal Antibody (bs-0161R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


HepG2 cells were stained with p75 NGF receptor Polyclonal Antibody, Unconjugated(bs-0161R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C


Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.


Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NGF Receptor) polyclonal Antibody, Unconjugated (bs-0161R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei


Mouse spleen cells probed with p75 NGF Receptor Polyclonal Antibody, unconjugated (bs-0161R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)