bs-0162R [Primary Antibody]
iNOS Polyclonal Antibody
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Host: Rabbit

Target Protein: iNOS

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18126

Source: KLH conjugated synthetic peptide derived from human NOS-2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
ICC/IF(1:50-100)

Cross Reactive Species: Human
Mouse
Rat
Dog
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hayakawa, Kentaro, et al. "Lipopolysaccharide preconditioning facilitates M2 activation of resident microglia after spinal cord injury." Journal of Neuroscience Research (2014).Read more>>
  • Zhang et al. High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats. (2015) Int.J.Clin.Exp.Patho. 8:5189-95Read more>>
  • Shanshan et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. (2017) Front.Cell.Neurosc. 11:99Read more>>
  • Wang et al. CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. (2018) Cell.Death.Dis. 9:880Read more>>
  • Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437.Read more>>
  • Lv R et al. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. Int Immunopharmacol. 2019 Feb 18;70:28-36.Read more>>
  • Imoto K et al. Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide SynthaseExpression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Int J Mol Sci. 2018 Dec 24;20(1). pii: E62.Read more>>
  • Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.Read more>>
  • "Guo MH et al. Elemental imbalance elicited by arsenic and copper exposure lead to oxidative stress and immunotoxicity in chicken gizzard, activating the protective effects of heat shock proteins. Environmental Science and Pollution Research. 2019."Read more>>
  • Huang P et al. Theoretical basis of nitrosomyoglobin formation in a dry sausage model by coagulase-negative staphylococci: Behavior and expression of nitric oxide synthase. Meat Sci. 2019 Nov 27;161:108022.Read more>>
  • Jiao F et al. AGK2 Alleviates Lipopolysaccharide Induced Neuroinflammation through Regulation of Mitogen-Activated Protein Kinase Phosphatase-1. J Neuroimmune Pharmacol. 2019 Nov 30. Read more>>
  • Wang M et al. Berberine combined with cyclosporine A alleviates acute graft-versus-host disease in murine models. Int Immunopharmacol. 2020 Feb 9;81:106205.Read more>>
  • El-Lakany MA et al. Ovariectomy provokes inflammatory and cardiovascular effects of endotoxemia in rats: Dissimilar benefits of hormonal supplements. Toxicol Appl Pharmacol. 2020 Feb 21;393:114928.Read more>>
  • Jiang Q et al. Particulate Matter 2.5 Induced Developmental Cardiotoxicity in Chicken Embryo and Hatchling. Front Pharmacol. 2020 Jun 5;11:841.Read more>>
  • Yang F et al. The effects of binge alcohol exposure on tooth movement and associated root resorption in rats. Alcohol. 2020 Nov;88:1-9.Read more>>
  • Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240.Read more>>
  • Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919Read more>>
  • Xue Wang. et al. Engineered liposomes targeting the gutCCNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:39Read more>>
  • Qian Xueshen. et al. Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimers Disease. J Alzheimers Dis. 2021 Jan;79(4):1785-18Read more>>
  • Yinying Sheng. et al. Development and In Vitro Biodegradation of Biomimetic Zwitterionic Phosphorylcholine Chitosan Coating on Zn1Mg Alloy. Acs Appl Mater Inter. 2020;12(49):54445C54458Read more>>
  • Liu Tang. et al. Topical Application with Conjugated Linoleic Acid Ameliorates 2, 4\dinitrofluorobenzene\Induced Atopic Dermatitis\like Lesions in BALB/c Mice. Exp Dermatol. 2021 Feb;30(2):237-248Read more>>
  • Sawada Noritaka. et al. Glucagon-Like Peptide-1 Receptor Agonist Liraglutide Ameliorates the Development of Periodontitis. J Diabetes Res. 2020;2020:884331Read more>>
  • Shuai Chen. et al. Chondroitin sulfate modified 3D porous electrospun nanofiber scaffolds promote cartilage regeneration. Mat Sci Eng C-Mater. 2021 Jan;118:111312Read more>>
  • Penghui Zhang. et al. Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration. Bioact Mater. 2021 Oct;6:3097Read more>>
  • Kai Kang. et al. FGF21 alleviates chronic inflammatory injury in the aging process through modulating polarization of macrophages. Int Immunopharmacol. 2021 Jul;96:107634Read more>>
  • Li Liu. et al. Inhibition of inducible nitric oxide synthase improved erectile dysfunction in rats with type 1 diabetes. 2021 Jun 16Read more>>
  • Guanglin Zhang. et al. Soft apoptotic-cell-inspired nanoparticles persistently bind to macrophage membranes and promote anti-inflammatory and pro-healing effects. Acta Biomater. 2021 JulRead more>>
  • Fangyu Qiao. et al. 4-Octyl itaconate modified demineralized bone matrix scaffold improves bone repair by regulating early inflammation. Chem Eng J. 2021 Dec;425:13149Read more>>
  • Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359Read more>>
  • Xueyang Zhao. et al. Improved tribological properties, cyto-biocompatibility and anti-inflammatory ability of additive manufactured Ti-6Al-4V alloy through surface texturing and nitriding. Surf Coat Tech. 2021 Nov;425:127686Read more>>
  • ingcheng Yi. et al. Lysine-doped polydopamine coating enhances antithrombogenicity and endothelialization of an electrospun aligned fibrous vascular graft. Appl Mater Today. 2021 Dec;25:101198Read more>>
  • Xin-yu Zhang. et al. Cerebralcare Granule? combined with nimodipine improves cognitive impairment in bilateral carotid artery occlusion rats by reducing lipocalin-2. Life Sci. 2021 Dec;286:120048Read more>>
  • Hu, Shuang. et al. Deletion of p38 attenuates ethanol consumption- and acetaminophen-induced liver injury in mice through promoting Dlg1. Acta Pharmacol Sin. 2021 Nov;:1-16Read more>>
  • Yun-Jiao Shi. et al. Impact of the Plastein Reaction of Casein Hydrolysates in the Presence of Exogenous Amino Acids on Their Anti-Inflammatory Effect in the Lipopolysaccharide-Stimulated Macrophages. Foods. 2022 Jan;11(2):196Read more>>
  • Weifeng Li. et al. Schisandrin improves lipopolysaccharide-induced acute lung injury by inhibiting the inflammatory response in vivo and invitro. 2022 Mar 21Read more>>
  • Hao Chen. et al. Au nanocluster-modulated macrophage polarization and synoviocyte apoptosis for enhanced rheumatoid arthritis treatment. J MATER CHEM B. 2022 MayRead more>>
  • Xin Zhao. et al. Diammonium glycyrrhizinate ameliorates portal hypertension by regulating portal macrophage oxidation and superoxide dismutase 3. EUR J PHARMACOL. 2022 Jun;:175115Read more>>
  • ingcheng Yi. et al. Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing. BIOACT MATER. 2022 JulRead more>>
  • Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683Read more>>
  • Guirong Chen. et al. The interaction of MD-2 with small molecules in huanglian jiedu decoction play a critical role in the treatment of sepsis.. FRONT PHARMACOL. 2022 Sep;13:947095-947095Read more>>
  • Sisi Lei. et al. Chaihu Shugan powder alleviates liver inflammation and hepatic steatosis in NAFLD mice: A network pharmacology study and <i>in vivo</i> experimental validation.. FRONT PHARMACOL. 2022 Sep;13:967623-967623Read more>>
  • Congyang Mao. et al. Realising highly efficient sonodynamic bactericidal capability through the phononCelectron coupling effect using two-dimensional catalytic planar defects. ADV MATER. 2022 Dec;:2208681Read more>>
  • Chuang Yang. et al. Gastrodin protects endothelial cells against high glucose-induced injury through up-regulation of PPAR and alleviation of nitrative stress. MICROVASC RES. 2023 Jul;148:104531Read more>>
  • Fangyu Qiao. et al. Hybrid Cell Membrane-Functionalized Matrixes for Modulating Inflammatory Microenvironment and Improving Bone Defect Repair. ADV HEALTHC MATER. 2023 Apr;:2203047Read more>>
  • Jiale Chen. et al. H2S-releasing versatile hydrogel dressing with potent antimicrobial, anti-inflammatory, epithelialization and angiogenic capabilities for diabetic wound healing. CHEM ENG J. 2023 Aug;469:143985Read more>>
  • Yang Liu. et al. Paeoniflorin Coordinates Macrophage Polarization and Mitigates Liver Inflammation and Fibrogenesis by Targeting the NF-B/HIF-1 Pathway in CCl4-Induced Liver Fibrosis. AM J CHINESE MED. 2023 Jun 14Read more>>
  • Shuhui Yu. et al. Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus. MOLECULES. 2023 Jan;28(12):4796Read more>>
  • Yonghong Zhang. et al. Decidual macrophages derived NO downregulates PD-L1 in trophoblasts leading to decreased Treg cells in recurrent miscarriage. FRONT IMMUNOL. 2023; 14: 1180154Read more>>
  • Qi Qi Pang. et al. Protective Effects and Mechanisms of Pectolinarin against H2O2-Induced Oxidative Stress in SH-SY5Y Neuronal Cells. MOLECULES. 2023 Jan;28(15):5826Read more>>
  • Qicai Liu. et al. Adropin deficiency worsens TNBS-induced colitis. INT IMMUNOPHARMACOL. 2023 Nov;124:110891Read more>>
VALIDATION IMAGES

Lane 1: rat lung lysates Lane 2: rat brain lysates probed with Anti NOS-2/iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 130kD. Observed band size: 115,130kD.


Lane 1: Mouse Raw264.7 tissue lysates; Lane 2: Human Jurkat cell lysates probed with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with iNOS polyclonal antibody, unconjugated (bs-0162R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pyogenic appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.