Host: Rabbit
Target Protein: BACE1
Immunogen Range: 401-501/501
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 23621
Swiss Prot: P56817
Source: KLH conjugated synthetic peptide derived from human BACE1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 51
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Pig
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Tian, Yue, et al. "Minocycline Alleviates Sevoflurane-Induced Cognitive Impairment in Aged Rats." Cellular and Molecular Neurobiology (2015): 1-10.Read more>>
- Hong, Yan, et al. "Effects of RAGE-Specific Inhibitor FPS-ZM1 on Amyloid-β Metabolism and AGEs-Induced Inflammation and Oxidative Stress in Rat Hippocampus." Neurochemical Research: 1-8.Read more>>
- Yan, Wei, et al. "NO2 inhalation promotes Alzheimer’s disease-like progression: cyclooxygenase-2-derived prostaglandin E2 modulation and monoacylglycerol lipase inhibition-targeted medication." Scientific Reports 6 (2016): 22429.Read more>>
- Jiao, Yanan, et al. "Osthole decreases beta amyloid levels through up-regulation of miR-107 in Alzheimer's disease." Neuropharmacology (2016).Read more>>
- Ku, Tingting, et al. "NF-κB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM 2.5 aspiration." Particle and Fibre Toxicology 14.1 (2017): 34.Read more>>
- Yuan C et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing β-amyloid clearance in APP/PS1 mice.(2019) Biochim Biophys Acta Mol Basis Dis.Jan;1865(1):161-180.Read more>>
- Lei Jiang. et al. Advanced glycation end products induce A142 deposition and cognitive decline through H19/miR-15b/BACE1 axis in diabetic encephalopathy. BRAIN RES BULL. 2022 Oct;188:187Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BACE1/ASP2 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates probed with Anti BACE1/ASP2 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 51kD. Observed band size:51kD.\n
Mouse muscle lysates probed with Rabbit Anti-BACE1 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BACE1 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HUVEC cell lysates probed with BACE1 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (BACE1) polyclonal Antibody, Unconjugated (bs-0164R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.