bs-0177R [Primary Antibody]
RAGE Polyclonal Antibody
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Host: Rabbit

Target Protein: RAGE

Immunogen Range: 151-250/403


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 81722

Swiss Prot: Q63495

Source: KLH conjugated synthetic peptide derived from rat AGER

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S1A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides (By similarity). Interaction with S1B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu, Wei, et al. "Curcumin Alleviates Diabetic Cardiomyopathy in Experimental Diabetic Rats." PloS one 7.12 (2012): e52013.Read more>>
  • Huang, Li-feng, et al. "The effect of high-mobility group box 1 protein on activity of regulatory T cells after thermal injury in rats." Shock 31.3 (2009): 322.Read more>>
  • Braach, Natascha, et al. "Anti-Inflammatory Functions of Protein C Require RAGE and ICAM-1 in a Stimulus-Dependent Manner." Mediators of Inflammation 2014 (2014).Read more>>
  • Zhu, Xiao-mei, et al. "Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function." Acta Pharmacologica Sinica (2014).Read more>>
  • Zhu, Xiao-mei, et al. "Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function." Acta Pharmacologica Sinica (2014).Read more>>
  • Sun, Yan‐Na, et al. "Effects of insulin combined with idebenone on blood–brain barrier permeability in diabetic rats." Journal of Neuroscience Research (2014).Read more>>
  • Fu, Juanli, et al. "Tetrachlorobenzoquinone exerts neurological pro-inflammatory activity by promoting HMGB1 release, which induces TLR4 clustering within the lipid raft." Toxicological Sciences (2016): kfw124.Read more>>
  • Sun et al. Expression and Significance of High-Mobility Group Protein B1 (HMGB1) and the Receptor for Advanced Glycation End-Product (RAGE) in Knee Osteoarthritis. (2016) Med.Sci.Monit. 22:2105-12Read more>>
  • Son et al. Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression. (2017) Sci.Rep. 7:270Read more>>
  • Huang J et al. Inhibition of the receptor for advanced glycation inhibits lipopolysaccharide-mediated Highmobility group protein B1 and Interleukin-6 synthesis in human gingival fibroblasts through the NF-κB signaling pathway. Arch Oral Biol. 2019 Sep;105:81-87. Read more>>
  • Gao R et al. Quasi-ultrafine particles promote cell metastasis via HMGB1-mediated cancer cell adhesion. Environ Pollut. 2019 Oct 23:113390. Read more>>
  • Chen Y et al. Dendritic cells-derived interferon-1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414.Read more>>
  • Kenjiro Hayashi. et al. Inhibitory Effects of Saururus chinensis Extract on Receptor for Advanced Glycation End-Products-Dependent Inflammation and Diabetes-Induced Dysregulation of Vasodilation. INT J MOL SCI. 2022 Jan;23(10):5757Read more>>
  • Aditi A. Joshi. et al. RAGE antagonism with azeliragon improves xenograft rejection by T cells in humanized mice.. CLIN IMMUNOL. 2022 Oct;:109165Read more>>
  • Zhitian Wang. et al. -hydroxybutyrate improves cognitive impairment caused by chronic cerebral hypoperfusion via amelioration of neuroinflammation and blood-brain barrier damage. BRAIN RES BULL. 2023 Feb;193:117Read more>>
  • Zheng-lan Duan. et al. Wumei Wan attenuates angiogenesis and inflammation by modulating RAGE signaling pathway in IBD: Network pharmacology analysis and experimental evidence. PHYTOMEDICINE. 2023 Mar;111:154658Read more>>
  • ajwa Seerat. et al. RAGE is a critical factor of sex-based differences in age-induced kidney damage. FRONT PHYSIOL. 2023 Mar;14:495Read more>>
  • Xuesong Zhang. et al. HMGB 1 acetylation mediates trichloroethylene-induced immune kidney injury by facilitating endothelial cell-podocyte communication. ECOTOX ENVIRON SAFE. 2023 Jul;259:115042Read more>>
  • Ola A. Habotta. et al. Sesquiterpene nootkatone counteracted the melamine-induced neurotoxicity via repressing of oxidative stress, inflammatory, and apoptotic trajectories. BIOMED PHARMACOTHER. 2023 Sep;165:115133Read more>>
  • Yufang Ma. et al. Apigenin and baicalein ameliorate thoracic aortic structural deterioration and cognitive deficit via inhibiting AGEs/RAGE/NF-B pathway in D-galactose-induced aging rats. EUR J PHARMACOL. 2024 Aug;976:17666Read more>>
VALIDATION IMAGES

Raji Cells lysates probed with RAGE Polyclonal Antibody, unconjugated (bs-0177R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Lane 1: Mouse Kidney lysates; Lane 2: Mouse Adrenal gland lysates; Lane 3: Rat Kidney lysates; Lane 4: Rat Adrenal gland lysates probed with RAGE Polyclonal Antibody, Unconjugated (bs-0177R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with RAGE polyclonal antibody, unconjugated (bs-0177R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ager | Rage Polyclonal Antibody, Unconjugated (bs-0177R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ager | Rage Polyclonal Antibody, Unconjugated (bs-0177R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ager | Rage Polyclonal Antibody, Unconjugated (bs-0177R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ager | Rage Polyclonal Antibody, Unconjugated (bs-0177R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.