bs-0189R
[Primary Antibody]
alpha Actin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: alpha Actin
Immunogen Range: 301-375/375
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 58
Source: KLH conjugated synthetic peptide derived from human alpha Actin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
Dog
Others
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Chicken
Cat
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722.Read more>>
- Liu, Yang, et al. "A Simple Method for Isolating and Culturing the Rat Brain Microvascular Endothelial Cells." Microvascular Research (2013).Read more>>
- Waladali, Wafi, et al. "17β-Estradiol affects the proliferation and apoptosis of rat bladder neck smooth muscle cells by modulating cell cycle transition and related proteins." World journal of urology 27.2 (2009): 241-248.Read more>>
- Song, Jin-Ning, et al. "Potential contribution of SOCC to cerebral vasospasm after experimental subarachnoid hemorrhage in rats." Brain Research (2013).Read more>>
- Han, Xiao, Jian-xun Liu, and Xin-zhi Li. "Salvianolic acid B inhibits autophagy and protects starving cardiac myocytes." Acta Pharmacologica Sinica 32.1 (2010): 38-44.Read more>>
- Lv, Ming-ming, Xin-dong Fan, and Li-xin Su. "Is a Swine Model of Arteriovenous Malformation Suitable for Human Extracranial Arteriovenous Malformation? A Preliminary Study." Cardiovascular and interventional radiology (2013): 1-7.Read more>>
- Guo, Jun, and Changting Liu. "Protective Effects of Telmisartan in a Rat Model of Pulmonary Arterial Hypertension." Experimental & Clinical Cardiology (2014).Read more>>
- Chen, Cheng-Hsien, et al. "MicroRNA-328 Inhibits Renal Tubular Cell Epithelial-to-Mesenchymal Transition by Targeting the CD44 in Pressure-Induced Renal Fibrosis." PloS one 9.6 (2014): e99802.Read more>>
- Wang, Yiming, and Limin Liao. "Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue." BMC Urology 14.1 (2014): 69.Read more>>
- Sun et al. Integrated long non-coding RNA analyses identify novel regulators of epithelial-mesenchymal transition in the mouse model of pulmonary fibrosis. (2016) J.Cell.Mol.Me. 20:1234-46Read more>>
- Wang et al. Histone demethylase retinoblastoma binding protein 2 regulates the expression of α-smooth muscle actin and vimentin in cirrhotic livers. (2013) Braz.J.Med.Biol.Re. 46:739-45Read more>>
- Tang et al. The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury. (2013) Evid.Based.Complement.Alternat.Me. 2013:820695Read more>>
- Liu et al. A boswellic acid-containing extract ameliorates schistosomiasis liver granuloma and fibrosis through regulating NF-κB signaling in mice. (2014) PLoS.On. 9:e100129Read more>>
- Deng et al. Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh. (2015) Stem.Cells.In. 2015:350718Read more>>
- Zeng et al. Resveratrol improves prostate fibrosis during progression of urinary dysfunction in chronic prostatitis by mast cell suppression. (2018) Mol.Med.Rep. 17:918-924Read more>>
- Senoo et al. Geranylgeranylacetone attenuates fibrogenic activity and induces apoptosis in cultured human hepatic stellate cells and reduces liver fibrosis in carbon tetrachloride-treated mice. (2018) BMC.Gastroenterol. 18:34Read more>>
- Ling et al. Long noncoding RNA ENSRNOG00000037522 is involved in the podocyte epithelial‑mesenchymal transition in diabetic rats. (2018) Int.J.Mol.Med. 41:2704-2714Read more>>
- Pan et al. DNA Methylation of PTGIS Enhances Hepatic Stellate Cells Activation and Liver Fibrogenesis. (2018) Front.Pharmacol. 9:553Read more>>
- Zhang et al. Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells. (2018) Int.J.Mol.Med. 42:279-289Read more>>
- Wang Y et al. ASIC1a promotes high glucose and PDGF-induced hepatic stellate cell activation by inducing autophagy through CaMKKβ/ERK signaling pathway. (2018) Toxicol Lett. (18)31963-5Read more>>
- Deng T et al.Inhibition Effect of Phytoestrogen Calycosin on TGF-β₁-Induced Hepatic Stellate Cells Activation, Proliferation and Migration via Estrogen Receptor β. (2018) Can. J. Physiol. Pharmacol. Oct 12Read more>>
- Shi B et al.Protein kinase C inhibitor chelerythrine attenuates partial unilateral ureteral obstruction induced kidney injury in neonatal rats.(2019) Life Sci. Jan 1;216:85-91.Read more>>
- Yin X et al. Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Arch Biochem Biophys. 2019 Jul 15;669:1-10. Read more>>
- Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261. Read more>>
- "Hu NH et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. Journal of Ethnopharmacology.2019."Read more>>
- Xu D et al. The synergistic action of phosphate and interleukin-6 enhances senescence-associated calcification in vascular smooth muscle cells depending on p53. Mech Ageing Dev. 2019 Sep;182:111124.Read more>>
- Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
- Han B et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2019 Dec 23;259:113870.Read more>>
- "Gao Y et al. Ginsenoside Re inhibits vascular neointimal hyperplasia in balloon-injured carotid arteries through activating the eNOS/NO/cGMP pathway in rats Y Gao, CY Gao, P Zhu, SF Xu, YM Luo, J Deng… - Biomedicine & …, 2018Biomed Pharmacother. 2018 Oct;106:1091-1097. "Read more>>
- Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
- J-Y Zhaoet al. Upregulated miR-101 inhibits acute kidney injuryCchronic kidney disease transition by regulating epithelialCmesenchymal transition. Hum Exp Toxicol. 2020 Dec;39(12):1628-1638.Read more>>
- Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachlorideCinduced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204Read more>>
- Wang W et al. Bu-Shen-Huo-Xue Decoction Ameliorates Diabetic Nephropathy by Inhibiting Rac1/PAK1/p38 MAPK Signaling Pathway in High-Fat Diet/Streptozotocin-Induced Diabetic Mice. Front Pharmacol.2020 Dec 3;11:587663.Read more>>
- Lili Hou. et al. Fumonisin B1 induces nephrotoxicity via autophagy mediated by mTORC1 instead of mTORC2 in human renal tubule epithelial cells. Food Chem Toxicol. 2021 Mar;149:112037Read more>>
- Chenjing Wang. et al. Salidroside and isorhamnetin attenuate urotensin?II?induced inflammatory response <em>in?vivo</em> and <em>in?vitro</em>: Involvement in regulating the RhoA/ROCK II pathway. Oncol Lett. 2021 Apr;21(4):1-1Read more>>
- Xue Wang. et al. Engineered liposomes targeting the gutCCNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:39Read more>>
- Fang-tian Bu. et al. Circular RNA circPSD3 alleviates hepatic fibrogenesis by regulating the miR-92b-3p/Smad7 axis. Mol Ther-Nucl Acids. 2021 Mar;23:847Read more>>
- Fuhua Wang. et al. Neuregulin\1 alleviate oxidative stress and mitigate inflammation by suppressing NOX4 and NLRP3/caspase\1 in myocardial ischaemia\reperfusion injury. J Cell Mol Med. 2021 Feb;25(3):1783-1795Read more>>
- Chang-Ming Zhong. et al. MicroRNA-92a -mediated endothelial to mesenchymal transition controls vein graft neointimal lesion formation. Exp Cell Res. 2021 Jan;398:112402Read more>>
- Zhigang Zhang. et al. Inflammation response after the cessation of chronic arsenic exposure and post-treatment of natural astaxanthin in liver: potential role of cytokine-mediated cellCcell interactions. Food Funct. 2020 Oct;11(10):9252-9262Read more>>
- Ma Zitong. et al. Combined Extracts of Epimedii Folium and Ligustri Lucidi Fructus with Budesonide Attenuate Airway Remodeling in the Asthmatic Rats by Regulating Apoptosis and Autophagy. Evid-Based Compl Alt. 2020;2020:2319409Read more>>
- Wen-qian Jia. et al. CD73 regulates hepatic stellate cells activation and proliferation through Wnt/-catenin signaling pathway. Eur J Pharmacol. 2021 Jan;890:173667Read more>>
- Lili Hou. et al. Combined protective effects of icariin and selenomethionine on novel chronic tubulointerstitial nephropathy models in vivo and vitro. Brit J Nutr. 2021 Mar;:1-33Read more>>
- Hongmei You. et al. The miR-455-3p/HDAC2 axis plays a pivotal role in the progression and reversal of liver fibrosis and is regulated by epigenetics. Faseb J. 2021 Jul;35(7):e217Read more>>
- Junpei Zhang. et al. Knockdown of TRIM15 inhibits the activation of hepatic stellate cells. 2021 Jun 17Read more>>
- Wenwen Liu. et al. Effects of andrographolide on renal tubulointersticial injury and fibrosis. Evidence of its mechanism of action. Phytomedicine. 2021 Jul;:15365Read more>>
- Liu Lulu. et al. Hydrogen Sulfide Attenuates Angiotensin II-Induced Cardiac Fibroblast Proliferation and Transverse Aortic Constriction-Induced Myocardial Fibrosis through Oxidative Stress Inhibition via Sirtuin 3. Oxid Med Cell Longev. 2021;2021:9925771Read more>>
- Liu Lulu. et al. Hydrogen Sulfide Attenuates Angiotensin II-Induced Cardiac Fibroblast Proliferation and Transverse Aortic Constriction-Induced Myocardial Fibrosis through Oxidative Stress Inhibition via Sirtuin 3. Oxid Med Cell Longev. 2021;2021:9925771Read more>>
- Xiaohang Wang. et al. Screening and Identification of Key Genes for Activation of Islet Stellate Cell. Front Endocrinol. 2021; 12: 695467Read more>>
- Yuan-Yuan Wu. et al. LncRNA MEG3 reverses CCl4-induced liver fibrosis by targeting NLRC5. Eur J Pharmacol. 2021 Nov;911:174462Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded dog bladder labeled with Anti-Alpha-SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: dog bladder tissue labeled with Anti-Actin A/Alpha SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300, followed by conjugation to the secondary antibody.
Mouse Small Intestine labeled with Anti-Actin A/Alpha SMA Polyclonal Antibody, Unconjugated(bs-0189R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C
NIH/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with alpha Actin Polyclonal Antibody(bs-0189R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha Actin) Polyclonal Antibody, Unconjugated (bs-0189R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha Actin) Polyclonal Antibody, Unconjugated (bs-0189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha Actin) Polyclonal Antibody, Unconjugated (bs-0189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha Actin) Polyclonal Antibody, Unconjugated (bs-0189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha Actin) Polyclonal Antibody, Unconjugated (bs-0189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha Actin) Polyclonal Antibody, Unconjugated (bs-0189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha Actin) Polyclonal Antibody, Unconjugated (bs-0189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Rat Liver tissue lysates; Lane 2: Rat Urinary bladder tissue lysates; Lane 3: Mouse Stomach tissue Lysates; Lane 4: Human A549 cell Lysates probed with alpha smooth muscle Actin Polyclonal Antibody, Unconjugated (bs-0189R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.