bs-0190R [Primary Antibody]
PACAP-27/38 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PACAP-27/38

Immunogen Range: 132-169/176


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 116

Swiss Prot: P18509

Source: KLH conjugated synthetic peptide derived from human PACAP-27/38

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 19


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lu L et al. Beneficial Effects of Crocin against Depression via Pituitary Adenylate Cyclase-Activating Polypeptide. Biomed Res Int. 2020 Jun 24;2020:3903125.Read more>>
  • Hailou Zhang. et al. Synergistic effects of two natural compounds of iridoids on rapid antidepressant action by up-regulating hippocampal PACAP signaling. 2022 Mar 31Read more>>
  • Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398Read more>>
  • Han Xuke. et al. Effects of electroacupuncture on bladder dysfunction and the expression of PACAP38 in a diabetic rat model. FRONT PHYSIOL. 2022 Dec;13:2751Read more>>
  • Alice Meng. et al. mGluR5 in astrocytes in the ventromedial hypothalamus regulates PACAP neurons and glucose homeostasis. J NEUROSCI. 2023 JulRead more>>
VALIDATION IMAGES

Rat cerebrum lysates probed with PACAP-27/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Rat cerebrum lysates probed with PACAP-27/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.