bs-0195R [Primary Antibody]
CD31 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD31

Immunogen Range: 681-738/738


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5175

Swiss Prot: P16284

Source: KLH conjugated synthetic peptide derived from human CD31

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-69 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 78


Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig
Rabbit
Cow

Predicted Cross Reactive Species: Dog
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han, Fengxuan, et al. "Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF." Biomaterials (2013).Read more>>
  • Zhang, Hong, et al. "Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration." Biomaterials (2013).Read more>>
  • Lu, Cong-Xiao, et al. "Sulfated polymannuroguluronate, a novel anti-AIDS drug candidate, inhibits HIV-1 Tat-induced angiogenesis in Kaposi's sarcoma cells." Biochemical pharmacology 74.9 (2007): 1330-1339.Read more>>
  • Lv, Fang, et al. "Repeated Abortion Affects Subsequent Pregnancy Outcomes in BALB/c Mice." PloS one 7.10 (2012): e48384.Read more>>
  • Gao, Qian, et al. "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." Molecular biology reports 39.5 (2012): 5791-5804.Read more>>
  • Lee, Hye Sook, Ji Hyun Lee, and Jae Wook Yang. "Effect of porcine chondrocyte derived extracellular matrix on the pterygium in mouse model." Graefes Archive for Clinical and Experimental Ophthalmology (2014) 1-10.Read more>>
  • Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456.Read more>>
  • Choi, Byung Hyune, et al. "Inhibition of blood vessel formation by a chondrocyte-derived extracellular matrix." Biomaterials (2014).Read more>>
  • Lee, Hye Sook, et al. "Anti-neovascular effect of chondrocyte-derived extracellular matrix on corneal alkaline burns in rabbits." Graefes Archive for Clinical and Experimental Ophthalmology (2014): 1-11.Read more>>
  • Lee, Tao-Chen, et al. "Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells." PLOS ONE 9.11 (2014): e111390.Read more>>
  • Xiang, Dongxi, et al. "Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors." Theranostics (2015) 5(10): 1083-1097.Read more>>
  • Sun, Wei, et al. "Adipose-Derived Stem Cells Alleviate Radiation-Induced Muscular Fibrosis by Suppressing the Expression of TGF-1." (2015) Stem Cells International.Read more>>
  • Karaca, T., et al. "Effects of hyperthyroidism on expression of vascular endothelial growth factor (VEGF) and apoptosis in fetal adrenal glands."European Journal of Histochemistry 59.4 (2015).Read more>>
  • Struecker, B., et al. "Implantation of a Tissue-Engineered Neo-Bile Duct in Domestic Pigs." European Surgical Research 56.1-2 (2015): 61-75.Read more>>
  • Zhang, Jue-yu, et al. "Local application of paeonol prevents early restenosis: a study with a rabbit vein graft model." Journal of Surgical Research (2016).Read more>>
  • Wang, Li, et al. "Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model." Peptides (2017).Read more>>
  • Kanthilal, Manisha. Characterization of Molecular and Mechanical Phenotypes of Freshly Isolated Lipoaspirate Cells. Diss. Brown University, 2013.Read more>>
  • Korkmaz, H. Ibrahim, et al. "Neutrophil Extracellular Traps Coincide with a Pro‐coagulant Status of Microcirculatory Endothelium in Burn Wounds." Wound Repair and Regeneration (2017).Read more>>
  • Kong, Hyun Sun, et al. "Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice." PloS one 12.9 (2017): e0184546.Read more>>
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Li et al. Repair of lipopolysaccharide-induced acute lung injury in mice by endothelial progenitor cells, alone and in combination with simvastatin. (2013) Ches. 144:876-86Read more>>
  • Tan et al. Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds. (2011) Int.J.Nanomedicin. 6:929-42Read more>>
  • Ji et al. Sodium humate accelerates cutaneous wound healing by activating TGF-β/Smads signaling pathway in rats. (2016) Acta.Pharm.Sin.. 6:132-40Read more>>
  • Lee et al. Chondrocyte-derived extracellular matrix suppresses pathogenesis of human pterygium epithelial cells by blocking the NF-κB signaling pathways. (2017) Mol.Vi. 22:1490-1502Read more>>
  • Wu et al. Overexpression and correlation of HIF-2α, VEGFA and EphA2 in residual hepatocellular carcinoma following high-intensity focused ultrasound treatment: Implications for tumor recurrence and progression. (2017) Exp.Ther.Me. 13:3529-3534Read more>>
  • Pei et al. Plantamajoside, a potential anti-tumor herbal medicine inhibits breast cancer growth and pulmonary metastasis by decreasing the activity of matrix metalloproteinase-9 and -2. (2015) BMC.Cance. 15:965Read more>>
  • Yang et al. Tiamulin inhibits breast cancer growth and pulmonary metastasis by decreasing the activity of CD73. (2017) BMC.Cance. 17:255Read more>>
  • Wang et al. Targeting neurokinin-3 receptor: a novel anti-angiogenesis strategy for cancer treatment. (2017) Oncotarget. 8:40713-40723Read more>>
  • Tang et al. Botulinum toxin A improves adipose tissue engraftment by promoting cell proliferation, adipogenesis and angiogenesis. (2017) Int.J.Mol.Med. 40:713-720Read more>>
  • Tung et al. Fabrication of a reticular poly(lactide-co-glycolide) cylindrical scaffold for the in vitro development of microvascular networks. (2017) Sci.Technol.Adv.Mater. 18:163-171Read more>>
  • Taniguchi,et al.Scaffold-free trachea regeneration by tissue engineering with bio-3D printing.(2018) Interactive CardioVascular and Thoracic Surgery. 26:745-752.Read more>>
  • Connell,et al.Eliminating Regurgitation Reduces Fibrotic Remodeling of Functional Mitral Regurgitation Conditioned Valves.(2018) Annals of Biomedical Engineering. 46:670-683.Read more>>
  • Brumm et al. Astrocytes Can Adopt Endothelial Cell Fates in a p53-Dependent Manner. (2017) Mol.Neurobiol. 54:4584-4596Read more>>
  • Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757.Read more>>
  • Takeoka Y et al. Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing.PLoS One. 2019 Mar 8;14(3):e0211339. Read more>>
  • Taniguchi D et al. Human lung microvascular endothelial cells as potential alternatives to human umbilical vein endothelial cells in bio-3D-printed trachea-like structures. Tissue and Cell,2020, 63, 101321.Read more>>
  • Saribas GS et al. Effects of uterus derived mesenchymal stem cells and their exosomes on asherman's syndrome. Acta Histochem. 2019 Nov 24:151465.Read more>>
  • Zhang C et al. Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats. Nanomedicine. 2019 Aug 7;21:102078.Read more>>
  • Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
  • Jin S et al. Conditioned medium derived from FGF-2-modified GMSCs enhances migration and angiogenesis of human umbilical vein endothelial cells. Stem Cell Res Ther. 2020 Feb 18;11(1):68.Read more>>
  • Li S et al. The Effect of YiQiFuMai on Ischemic Heart Failure by Improve Myocardial Microcirculation and Increase eNOS and VEGF Expression. International Journal of Clinical Medicine,2020 11, 84-100.Read more>>
  • Hayashi T et al. Characteristics of Leiomyosarcoma: Induction of Hematogenous Metastasis by Isolated Uterine Mesenchymal Tumor Stem-like Cells Anticancer Res. 2020 Mar;40(3):1255-1265.Read more>>
  • Kai-Li Liu et al. Effects of seawater immersion on open traumatic brain injury in rabbit model. Brain Res. 2020 Sep 15;1743:146903.Read more>>
  • Fan Z et al. Novel pre-vascularized tissue-engineered dermis based on stem cell sheet technique used for dermis-defect healingRegen Biomater.2020 Oct 8;7(6):627-638.Read more>>
  • Weidong Jiang. et al. MicroRNA-205 mediates endothelial progenitor functions in distraction osteogenesis by targeting the transcription regulator NOTCH2. Stem Cell Res Ther. 2021 Dec;12(1):1-14Read more>>
  • Jie Mi. et al. Calcitonin Gene-Related Peptide Enhances Distraction Osteogenesis by Increasing Angiogenesis. 2020 Nov 02Read more>>
  • Pierre Jean Le Reste. et al. Local intracerebral inhibition of IRE1 by MKC8866 sensitizes glioblastoma to irradiation/chemotherapy in vivo. Cancer Lett. 2020 Dec;494:73Read more>>
  • Hannah Everwien. et al. Engineering an endothelialized, endocrine Neo-Pancreas: Evaluation of islet functionality in an ex vivo model. Acta Biomater. 2020 Nov;117:213Read more>>
  • Xuhua Duan. et al. Antitumor properties of arsenic trioxide-loaded CalliSpheres? microspheres by transarterial chemoembolization in VX2 liver tumor rabbits: suppression of tumor growth, angiogenesis, and metastasis and elongation of survival. Am J Transl Res. 2020; 12(9): 5511C5524Read more>>
  • Hao Cai. et al. Cathepsin B-responsive and gadolinium-labeled branched glycopolymer-PTX conjugate-derived nanotheranostics for cancer treatment. Acta Pharm Sin B. 2021 Feb;11:544Read more>>
VALIDATION IMAGES

Human U937 cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:20 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).


U937 lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:200 overnight at 4°C, DAB staining.