bs-0209R [Primary Antibody]
CD133 antigen Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD133 antigen

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8842

Swiss Prot: O43490

Source: KLH conjugated synthetic peptide derived from human CD133 antigen

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Predicted Molecular Weight: 95


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Fruit Fly

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shih-Ying Sung. et al. Supercritical carbon dioxide-decellularized arteries exhibit physiologic-like vessel regeneration following xenotransplantation in rats. BIOMATER SCI-UK. 2023 FebRead more>>
VALIDATION IMAGES

Lane 1: NIH/3T3 cell lysates; Lane 2: Lovo cell lysates; Lane 3: SW480 cell lysates probed with CD133 Polyclonal Antibody, Unconjugated (bs-0209R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.