bs-0216R [Primary Antibody]
Fas Ligand Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Fas Ligand

Immunogen Range: 196-281/281


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 356

Swiss Prot: P48023

Source: KLH conjugated synthetic peptide derived from human Fas Ligand

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Cui-Ying, et al. "Characterization of cytotoxicity-related gene expression in response to virulent Marek??s disease virus infection in the bursa of Fabricius."Research in veterinary science (2012).qRead more>>
  • Wang, Yi, et al. "Lipopolysaccharide‐induced expression of FAS ligand in cultured immature boar Sertoli cells through the regulation of pro‐inflammatory cytokines and miR‐187." Molecular Reproduction and Development (2015).Read more>>
  • Song, Xiufang, et al. "Polychlorinated biphenyl quinone metabolite promotes p53-dependent DNA damage checkpoint activation, S-phase cycle arrest and extrinsic apoptosis in human liver hepatocellular carcinoma HepG2 Cells."Chemical Research in Toxicology (2015).Read more>>
  • Wang, Shuhua, Qingqing Tian, and Fang An. "Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells." Oncology Letters (2016).Read more>>
  • Qi, Suqin, et al. "BPA-induced apoptosis of rat Sertoli cells through Fas/FasL and JNKs/p38 MAPK pathways." Reproductive Toxicology 50 (2014): 108-116.Read more>>
  • Shi, Yuqin, et al. "p, p′-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway." Journal of Biomedicine and Biotechnology (2009).Read more>>
  • Shi, Yuqin, et al. "β‐benzene hexachloride induces apoptosis of rat sertoli cells through generation of reactive oxygen species and activation of JNKs and FasL." Environmental toxicology 26.2 (2011): 124-135.Read more>>
  • Lv, Yanhong, et al. "Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma." Scientific Reports 7 (2017): 40967.Read more>>
  • Peng et al. Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells I n -v itro. (2015) Iran.J.Pharm.Re. 14:251-61Read more>>
  • Zhang et al. Cytochrome P450 3A1 mediates 2,2',4,4'-tetrabromodiphenyl ether-induced reduction of spermatogenesis in adult rats. (2013) PLoS.One. 8:e66301Read more>>
  • Liu S et al. PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK. Theriogenology. 2019 Jun;131:89-95.Read more>>
  • Jimbo H et al. Fas‐FasL interaction in cytotoxic T cell‐mediated vitiligo: The role of lesional expression of tumor necrosis factor‐α and interferon‐γ in Fas‐mediated melanocyte … Exp Dermatol. 2019 Nov 1.Read more>>
  • Wang Z et al. β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells.Oncol Lett. 2018 Oct;16(4):4519-4525.Read more>>
  • Yuefeng Gaoet al. Vitamin E promotes ovine Sertoli cell proliferation by regulation of genes associated with cell division and the cell cycle. Anim Biotechnol. 2020 Jul 2;1-9.Read more>>
  • Jisheng Wang. et al. Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway. Med Sci Monitor. 2021; 27: e928232-1Ce928232-12Read more>>
  • Janos L. Tanyi. et al. Personalized cancer vaccine strategy elicits polyfunctional T cells and demonstrates clinical benefits in ovarian cancer. Npj Vaccines. 2021 Mar;6(1):1-14Read more>>
  • Lai, Ting-Yu. et al. Different methods of detaching adherent cells and their effects on the cell surface expression of Fas receptor and Fas ligand. SCI REP-UK. Sci Rep-Uk. 2022 Apr;12(1):1-8Read more>>
  • Le Zhang. et al. Dioscin Regulating Bone Marrow Apoptosis in Aplastic Anemia. DRUG DES DEV THER. 2022 Sep;16:3041-3053Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Jurkat lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Bone lysates; Lane 2: Rat Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.