bs-0223R [Primary Antibody]
MEK2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MEK2

Immunogen Range: 1-50/400


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5605

Swiss Prot: P36507

Source: KLH conjugated synthetic peptide derived from human MAPKK2/MEK2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 46


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Images provided by the Independent Validation Program, badge number 029748. Lane one: HeLa cell lysates. Lane 2: c6/36 Mosquito cell extract (non-reactive\nspecies) probed with Rabbit Anti-MEK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.


HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250. Followed by Goat Anti-Rabbit antibody conjugated to Alexa fluor 488 at 1:500 dilution.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPKK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.