bs-0229R [Primary Antibody]
FGF1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FGF1

Immunogen Range: 16-80/155


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14164

Swiss Prot: P61148

Source: KLH conjugated synthetic peptide derived from mouse FGF1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Sheep

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti FGF1/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FGF1/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse heart lysates probed with Rabbit Anti-FGF1 Polyclonal Antibody, Unconjugated (bs-0229R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.