bs-0233R
[Primary Antibody]
TERT Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TERT
Immunogen Range: 601-750/1132
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7015
Swiss Prot: O14746
Source: KLH conjugated synthetic peptide derived from human TERT
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 125
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Carreras et al. Chronic sleep fragmentation induces endothelial dysfunction and structural vascular changes in mice. (2014) Slee. 37:1817-24Read more>>
- Esra Gozde Kosebent et al. The altered expression of telomerase components and telomere-linked proteins may associate with ovarian aging in mouse. Exp Gerontol. 2020 Sep;138:110975.Read more>>
- Li X et al. BDE-209 and DBDPE induce male reproductive toxicity through telomere-related cell senescence and apoptosis in SD ratEnviron Int.2021 Jan;146:106307.Read more>>
- Esra G. Kosebent. et al. The spatiotemporal expression of TERT and telomere repeat binding proteins in the postnatal mouse testes. 2021 Feb 05Read more>>
- Jiahuan Wu. et al. Cycloastragenol protects against glucocorticoid\induced osteogenic differentiation inhibition by activating telomerase. 2020 Nov 09Read more>>
- Fatma Uysal. et al. Decreased expression of TERT and telomeric proteins as human ovaries age may cause telomere shortening. J Assist Reprod Gen. 2021 Feb;38(2):429-441Read more>>
- Kosebent, Esra Gozde. et al. Telomere associated gene expression as well as TERT protein level and telomerase activity?are altered in the ovarian follicles of aged mice. Sci Rep-Uk. 2021 Jul;11(1):1-11Read more>>
- Xiaoyu Qi. et al. Curcumol inhibits ferritinophagy to restrain hepatocyte senescence through YAP/NCOA4 in non-alcoholic fatty liver disease. 2021 Aug 03Read more>>
- Dimitris Tsoukalas. et al. Reversal of brain aging by targeting telomerase: A nutraceutical approach. Int J Mol Med. 2021 Nov;48(5):1-11Read more>>
- Qi Xiaoyu. et al. Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3BLamin B1 Interaction in Alcoholic Fatty Liver Disease. FRONT PHARMACOL. 2022 Jun;0:214Read more>>
- Long Xie. et al. plePan-cancer analysis of TERT and validation in osteosarcoma cell lines. BIOCHEM BIOPH RES CO. 2022 NovRead more>>
- Yue Zhang. et al. Dietary selenium excess affected spermatogenesis via DNA damage and telomere-related cell senescence and apoptosis in mice. FOOD CHEM TOXICOL. 2023 Jan;171:113556Read more>>
- Ze-Rui Zhou. et al. DNAzyme-Powered DNA Walker for Cooperative Expression Imaging of Mutant p53 and Telomerase in Cancer Cells. ANAL CHEM. 2023;95(8):4122C413Read more>>
- Mingyue Ma. et al. Schisandrin B promotes senescence of activated hepatic stellate cell via NCOA4-mediated ferritinophagy. PHARM BIOL. 2023;61(1):621-629Read more>>
- Guangmou Zhang. et al. Silencing TCAB1 suppresses proliferation of hepatocellular carcinoma cells by inducing apoptosis. TURK J BIOCHEM. 2023 ApRead more>>
- Fangji Zhou. et al. Mono-(2-ethylhexyl) Phthalate (MEHP)-Induced Telomere Structure and Function Disorder Mediates Cell Cycle Dysregulation and Apoptosis via c-Myc and Its Upstream Transcription Factors in a Mouse Spermatogonia-Derived (GC-1) Cell Line. TOXICS. 2023 May;11(5):448Read more>>
- Meng-Ying Liu. et al. TERT mediates the U-shape of glucocorticoids effects in modulation of hippocampal neural stem cells and associated brain function. CNS NEUROSCI THER. 2024 Feb;30(2):e14577Read more>>
- Jialing Sun. et al. Investigating the molecular mechanism of Qizhu anticancer prescription in inhibiting hepatocellular carcinoma based on high-resolution mass spectrometry and network pharmacology. J ETHNOPHARMACOL. 2024 Feb;:117985Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human hepatoma labeled with Anti-TRT/TERT Polyclonal Antibody, Unconjugated (bs-0233R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TERT Polyclonal Antibody, Unconjugated (bs-0233R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TERT Polyclonal Antibody, Unconjugated (bs-0233R) at 1:200 overnight at 4°C, DAB staining.
A375 lysates probed with TERT Polyclonal Antibody, Unconjugated (bs-0233R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Thymus lysates probed with TERT Polyclonal Antibody, Unconjugated (bs-0233R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TERT Polyclonal Antibody(bs-0233R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (TERT) polyclonal Antibody, Unconjugated (bs-0233R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.