bs-0255R [Primary Antibody]
Estrogen receptor beta Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Estrogen receptor beta

Immunogen Range: 50-100/530


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2100

Swiss Prot: Q92731

Source: KLH conjugated synthetic peptide derived from human ER-Beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhou, Dawei, et al. "Estrogen receptor alpha is essential for the proliferation of prostatic smooth muscle cells stimulated by 17β‐estradiol and insulin‐like growth factor 1." Cell biochemistry and function 29.2 (2011): 120-125.Read more>>
  • Wu et al. Downregulation of G?protein?coupled receptor 30 in the hippocampus attenuates the neuroprotection of estrogen in the critical period hypothesis. (2018) Mol.Med.Rep. 17:5716-5725Read more>>
  • Jialing Sun. et al. Investigating the molecular mechanism of Qizhu anticancer prescription in inhibiting hepatocellular carcinoma based on high-resolution mass spectrometry and network pharmacology. J ETHNOPHARMACOL. 2024 Feb;:117985Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human brain tissue labeled with Rabbit Anti-ER-Beta Polyclonal Antibody, Unconjugated (bs-0255R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Mouse Ovary tissue lysates; Lane 4: Mouse Uterus tissue lysates; Lane 5: Rat Uterus tissue Lysates probed with Estrogen receptor beta Polyclonal Antibody, Unconjugated (bs-0255R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.