bs-0279R [Primary Antibody]
VEGF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VEGF

Immunogen Range: 27-77/232

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7422

Swiss Prot: P15692

Source: KLH conjugated synthetic peptide derived from human VEGF

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ryzhov, Sergey, et al. "Role of a2b adenosine receptors in regulation of paracrine functions of stem cell antigen 1-positive cardiac stromal cells." Journal of Pharmacology and Experimental Therapeutics 341.3 (2012): 764-774.Read more>>
  • Zeng, K.-W., et al., Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis. Eur J Pharmacol (2013)Read more>>
  • Li, Junqin, et al. "Fatty Acid Synthase Mediates the Epithelial-Mesenchymal Transition of Breast Cancer Cells." International Journal of Biological Sciences 10.2 (2014): 171-180.Read more>>
  • Zhang, Q. L., et al. "Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of liaoning cashmere goats." Genet Mol Res 12.12 (2013): 6424-6432.Read more>>
  • Qi, Yuanyuan, et al. "Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells." PloS one 7.3 (2012): e33859.Read more>>
  • Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).Read more>>
  • Guo, Jian-wen, et al. "Combinatorial effects of Naomai Yihao Capsules () and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tissues in rats." Journal of Traditional Chinese Medicine 32.1 (2012): 87-92.Read more>>
  • Chen et al. Circulating fibrocyte mobilization in negative pressure wound therapy. (2017) J.Cell.Mol.Me. 21:1513-1522Read more>>
  • Chen et al. Circulating fibrocyte mobilization in negative pressure wound therapy. (2017) J.Cell.Mol.Me. 21:1513-1522Read more>>
  • Yasukawa et al. Synergetic Effects of PARP Inhibitor AZD2281 and Cisplatin in Oral Squamous Cell Carcinoma in Vitro and in Vivo. (2016) Int.J.Mol.Sc. 17:272Read more>>
  • Wang et al. Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage. (2012) Neural.Regen.Res. 7:595-600Read more>>
  • Bi et al. Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice. (2017) Sci.Rep. 7:10001Read more>>
  • Chen et al. Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair. (2018) Exp.Ther.Med. 16:821-829Read more>>
  • Yao Q et al.Lycium Barbarum Polysaccharides Improve Retinopathy in Diabetic Sprague-Dawley Rats.(2018)Evid Based Complement Alternat Med.Nov 15;2018:7943212. Read more>>
  • Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55.Read more>>
  • Fernandez-Bueno I et al. Reliability and reproducibility of a rodent model of choroidal neovascularization based on the subretinal injection of polyethylene glycol.Mol Vis. 2019 Mar 18;25:194-203. Read more>>
  • Varghese S et al. Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Int Immunopharmacol. 2019 May 21;73:280-292.Read more>>
  • Wang J et al. A Novel RNA Aptamer-modified Riboswitch as Chemical sensor. Analytica Chimica Acta. (2019).Read more>>
  • W Jiang et al. EFFECTS OF MESENCHYMAL STEM CELL TRANSPLANTATION INTO ISCHEMIC BILE DUCT INJURY TISSUES OF RATS ON THE EXPRESSION OF VASCULAR DENSITY. Slov Vet Res 2019: 56 (4): 183–90.Read more>>
  • Zheng W et al. An injectable thermosensitive hydrogel for sustained release of apelin-13 to enhance flap survival in rat random skin flap. J Mater Sci Mater Med. 2019 Sep 9;30(9):106. Read more>>
  • Mei F et al. Collagen peptides isolated from Salmon salar and Tilapia nilotica skin accelerate wound healing by altering cutaneous microbiome colonization via up-regulated NOD2 and BD14. Agric. Food Chem. 2020, 68, 6, 1621-1633.Read more>>
  • Sun Y et al. The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism. J Cancer Res Clin Oncol. 2020 May;146(5):1139-1152. Read more>>
VALIDATION IMAGES

Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 23,46kDa


Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated.\n