bs-0289R [Primary Antibody]
Neurofascin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Neurofascin

Immunogen Range: 501-650/1347


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23114

Swiss Prot: O94856

Source: KLH conjugated synthetic peptide derived from human Neurofascin-155

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 132/150


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded: rat spinal cord tissue labeled with Anti-Neurofascin Polyclonal Antibody, Unconjugated (bs-0289R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Neurofascin Polyclonal Antibody, Unconjugated (bs-0289R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Mouse cerebellum lysates probed with Neurofascin Polyclonal Antibody, unconjugated (bs-0289R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Rat Cerebellum lysates probed with Neurofascin Polyclonal Antibody, Unconjugated (bs-0289R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.