bs-0293G [Secondary Antibody]
Goat Anti-Rat IgG Antibody (H+L)
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Goat

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Lyophilized

Storage: Store at -20°C for 12 months.

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Size: 1mg

Concentration: Determined by end user

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Yun, et al. "Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection." Infection and Immunity 80.8 (2012): 2948-2955Read more>>
  • Hu, Yong-hua, et al. "Construction and evaluation of a live vaccine against< i> Edwardsiella tarda</i> and< i> Vibrio harveyi</i>: Laboratory vs. mock field trial." Vaccine 29.24 (2011): 4081-4085.Read more>>
  • Cheng, Shun-feng, et al. "A single immunoglobulin-domain IgSF protein from< i> Sciaenops ocellatus</i> regulates pathogen-induced immune response in a negative manner." Developmental & Comparative Immunology 38.1 (2012): 117-127.Read more>>
  • Qiu, Reng, et al. "Identification and characterization of a cell surface scavenger receptor cysteine-rich protein of Sciaenops ocellatus: bacterial interaction and its dependence on the conserved structural features of the SRCR domain." Fish & shellfish immunology (2013).Read more>>
  • Sun, Yun, Chun-sheng Liu, and Li Sun. "A multivalent killed whole-cell vaccine induces effective protection against< i> Edwardsiella tarda</i> and< i> Vibrio anguillarum</i>." Fish & Shellfish Immunology 31.4 (2011): 595-599.Read more>>
  • Dang, Wei, Min Zhang, and Li Sun. "< i> Edwardsiella tarda</i> DnaJ is a virulence-associated molecular chaperone with immunoprotective potential." Fish & shellfish immunology 31.2 (2011): 182-188.Read more>>
  • Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene." Life Sci J 2013;10(3):963-969Read more>>
  • Tu, Q., et al. "Atorvastatin protects against cerebral ischemia/reperfusion injury through anti-inflammatory and antioxidant effects." Neural Regeneration Research 9.3 (2014): 268.Read more>>
  • Li, Mo-fei, et al. "Sil: A Streptococcus iniae Bacteriocin with Dual Role as an Antimicrobial and an Immunomodulator That Inhibits Innate Immune Response and Promotes S. iniae Infection." PLOS ONE 9.4 (2014): e96222.Read more>>
  • Zhang, J., Zhang, M., Sun, L.,"Junctional adhesion molecule A of red drum (Sciaenops ocellatus): a possible immunomodulator and a target for bacterial immune evasion." Veterinary Immunology and Immunopathology (2014)Read more>>
  • Wang, Xuguang, and Zhe Chen. "MicroRNA-19a functions as an oncogenic microRNA in non-small cell lung cancer by targeting the suppressor of cytokine signaling 1 and mediating STAT3 activation." International journal of molecular medicine 35.3 (2015): 839-846.Read more>>
  • Wang, S. M., et al. "A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting." Clinical, Cosmetic and Investigational Dermatology 8 (2015): 193-204.Read more>>
  • Zhang, Jian, and Mo-fei Li. "ORF75 of megalocytivirus RBIV-C1: a global transcription regulator and an effective vaccine candidate." Fish & Shellfish Immunology (2015).Read more>>
  • Zhong, Jiaju, et al. "A Smart Polymeric Platform for Multistage Nucleus-Targeted Anticancer Drug Delivery." Biomaterials (2015).Read more>>
  • Li, Lian, et al. "Multistage Nanovehicle Delivery System Based on Stepwise Size Reduction and Charge Reversal for Programmed Nuclear Targeting of Systemically Administered Anticancer Drugs." Advanced Functional Materials(2015).Read more>>
  • Zhang, Bao-cun, and Li Sun. "Tongue sole (Cynoglossus semilaevis) prothymosin alpha: cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and–independent pathways respectively." Developmental & Comparative Immunology (2015).Read more>>
  • Hou, Shuai, et al. "Mechanism of Mitochondrial Connexin43′ s Protection of the Neurovascular Unit under Acute Cerebral Ischemia-Reperfusion Injury." International Journal of Molecular Sciences 17.5 (2016): 679.Read more>>
  • Zhang, Haolong, et al. "Pleiotropic Effects of Survivin in Vascular Endothelial Cells." Microvascular Research (2016).Read more>>
  • Yan et al. Inflammation and pathological damage to the lungs of mice are only partially reversed following smoking cessation on subacute exposure to cigarette smoke. (2015) Mol.Med.Re. 11:4246-54Read more>>
  • Zhou and Sun Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival. (2016) Front.Cell.Infect.Microbiol. 6:76Read more>>
  • Zhou and Sun Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival. (2016) Front.Cell.Infect.Microbiol. 6:76Read more>>
  • Yu et al. Significance of multidrug resistance gene-related proteins in the postoperative chemotherapy of gastric cancer. (2015) Int.J.Clin.Exp.Pathol. 7:7945-50Read more>>
  • Zhang et al. P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. (2015) PLoS.One. 10:e0121282Read more>>
  • Sun and Sun CsBAFF, a Teleost B Cell Activating Factor, Promotes Pathogen-Induced Innate Immunity and Vaccine-Induced Adaptive Immunity. (2015) PLoS.One. 10:e0136015Read more>>
  • Liu B et al. Zhen-wu-tang ameliorates membranous nephropathy rats through inhibiting NF-κB pathway and NLRP3 inflammasome. Phytomedicine. 2019 Apr 2;59:152913. Read more>>
  • Yuan H et al. Calhex231 alleviates high glucose-induced myocardial fibrosis via inhibiting Itch-ubiquitin proteasome pathway in Vitro. Biol Pharm Bull. 2019 Jun 4. Read more>>
  • Bai, L et al. Renoprotective Effects of Artemisinin and Hydroxychloroquine Combination Therapy on IgA Nephropathy via Suppressing NF-κB Signaling and NLRP3 Inflammasome Activation by Exosomes in Rats. Biochemical Pharmacology. 2019 Aug22.Read more>>
  • Chen X et al. Early Therapeutic Vaccination Prediction of Hepatocellular Carcinoma via Imaging OX40 Mediated Tumor Infiltrating Lymphocytes. Mol Pharm. 2019 Aug 27. Read more>>
  • Wang Y et al. Tissue Targeting and Ultrasound-Targeted Microbubble Destruction Delivery of Plasmid DNA and Transfection In Vitro. Cellular and Molecular Bioengineering.2019Sep.Read more>>
  • Wang Y et al. Tissue Targeting and Ultrasound-Targeted Microbubble Destruction Delivery of Plasmid DNA and Transfection In Vitro. Cellular and Molecular Bioengineering.2019Sep.Zhang Y et al. Angiotensin Ⅱ deteriorates advanced atherosclerosis by promoting MerTK cleavage and impairing efferocytosis through AT1R/ROS/p38MAPK/ADAM17 pathway. Am J Physiol Cell Physiol. 2019 Aug 7.Read more>>
  • Suhaimi H et al. Identification of secretion domain of Neospora caninum profilin. Biochem Biophys Res Commun. 2019 Nov 14. pii: S0006-291X(19)32176-X.Read more>>
  • Wassie T et al. Recombinant B2L and Kisspeptin-54 DNA Vaccine Induces Immunity Against Orf Virus and Inhibits Spermatogenesis In Rats. Sci Rep. 2019 Nov 7;9(1):16262.Read more>>
  • Liu D et al. Effects of inhalation of sevoflurane at different concentrations on TRPV1 in airways of rats at different developmental stages. Life Sci. 2020 May 15;249:117472.Read more>>
  • Chen X et al. Hydrogel-based microbeads for Raman-encoded suspension array using the reversed-phase suspension polymerization method and ultraviolet light curing. Anal Bioanal Chem. 2020 May;412(12):2731-2741. Read more>>
  • Wang B et al. Laminin-dependent integrin β1 signaling regulates milk protein synthesis via prolactin/STAT5 pathway in bovine mammary epithelial cells. Biochem Biophys Res Commun. 2020 Jan 24. Read more>>
  • Iwaki T et al. Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr Purif. 2020 Feb 13;170:105607.Read more>>
  • Zhao Y et al. Interaction between regulatory T cells and mast cells via IL?9 and TGF?&beta; productionOncol Lett.2020 Dec;20(6):360.Read more>>
  • Zhao P et al. Rae1 drives NKG2D binding\dependent tumor development in mice by activating mTOR and STAT3 pathways in tumor cells. Cancer Sci. 2020 Jul;111(7):2234-2247.Read more>>
  • Quanyu Chen. et al. Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. Int Immunopharmacol. 2021 Jan;90:10718Read more>>
  • Zongyi Zhan. et al. Reduced Dendritic Spines in the Visual Cortex Contralateral to the Optic Nerve Crush Eye in Adult Mice. Invest Ophth Vis Sci. 2020 Aug;61(10):55-55Read more>>
  • Ling Kong. et al. Tg ROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection. Parasite Vector. 2020 Dec;13(1):1-14Read more>>
  • Nan Cheng. et al. B7-H3 augments the pro-angiogenic function of tumor-associated macrophages and acts as a novel adjuvant target for triple-negative breast cancer therapy. Biochem Pharmacol. 2021 Jan;183:114298Read more>>
  • Feng Tang. et al. Impact of beta-2 microglobulin expression on the survival of glioma patients via modulating the tumor immune microenvironment. 2021 May 07Read more>>
  • Ting-Ting Yu. et al. Macrophages mediated delivery of chlorin e6 and treatment of lung cancer by photodynamic reprogramming. Int Immunopharmacol. 2021 Nov;100:108164Read more>>
  • Zhang-Jie Yang. et al. SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in?vitro and in?vivo analyses. Pharm Biol. 2021;59(1):1314-1325Read more>>
  • Xin Peng. et al. The mitochondrial antioxidant SS-31 attenuated lipopolysaccharide-induced apoptosis and pyroptosis of nucleus pulposus cells via scavenging mitochondrial ROS and maintaining the stability of mitochondrial dynamics. 2021 Dec 14Read more>>
  • Xuejia Zhai. et al. The protective effect of hypericin on postpartum depression rat model by inhibiting the NLRP3 inflammasome activation and regulating glucocorticoid metabolism. Int Immunopharmacol. 2022 Apr;105:10856Read more>>
  • Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784Read more>>
  • Peng, Xin. et al. A20 attenuates pyroptosis and apoptosis in nucleus pulposus cells via promoting mitophagy and stabilizing mitochondrial dynamics. INFLAMM RES. 2022 Apr;:1-16Read more>>
  • Ma, Wenbin. et al. CircPCNX Promotes PDGF-BB-Induced Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cells Through Regulating miR-1278/DNMT1 Axis. CARDIOVASC DRUG THER. 2022 Jun;:1-13Read more>>