bs-0294D-Biotin [Conjugated Secondary Antibody]
Donkey Anti-Goat IgG Antibody (H+L), Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Donkey

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Goat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Song, Yuning, et al. "Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig." FEBS Open Bio (2016).Read more>>
  • Song, Zhiqi, et al. "REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling." Oncotarget 7.11 (2016): 12035.Read more>>
  • Luan, Xinhong, et al. "Comparative proteomic analysis of pituitary glands from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach." PLOS ONE 12.9 (2017): e0185253.Read more>>
  • Luan et al. Comparative proteomic analysis of pituitary glands from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach. (2017) PLoS.On. 12:e0185253Read more>>
  • Chen et al. Functional roles of C/EBPα and SUMO‑modification in lung development. (2017) Int.J.Mol.Me. 40:1037-1046Read more>>
  • Cui et al. Mycobacterium bovis Induces Endoplasmic Reticulum Stress Mediated-Apoptosis by Activating IRF3 in a Murine Macrophage Cell Line. (2016) Front.Cell.Infect.Microbiol. 6:182Read more>>
  • Liu et al. Induction of Fas mediated caspase-8 independent apoptosis in immune cells by Armigeres subalbatus saliva. (2012) PLoS.One. 7:e41145Read more>>
  • Deng Q et al. NLRP3 inflammasomes in macrophages drive colorectal cancer metastasis to the liver. (2018) Cancer Lett. Oct 28;442:21-30.Read more>>
  • Zhou J et al. Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS1 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic Inflammation Neurochemical Research.2019.Read more>>
  • Wang R et al. Role of astrocytes-derived D-serine in PFOS-induced neurotoxicity through NMDARs in the rat primary hippocampal neurons. Toxicology. 2019 Apr 17;422:14-24.Read more>>
  • Lei R et al. HMGB1 mediated autophagy protects glioblastoma cells from carbon-ion beam irradiation injury.Acta Astronautica. doi:10.1016/j.actaastro.2019.03.047 Read more>>
  • Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25.Read more>>
  • Zhang J et al. Gold-platinum nanoflowers as a label and as an enzyme mimic for use in highly sensitive lateralflow immunoassays: application to detection of rabbit IgG. Mikrochim Acta. 2019 May 16;186(6):357.Read more>>
  • Zhu G et al. Loss of PI3 kinase association improves the sensitivity of secondary mutation of KIT to Imatinib. Cell & Bioscience, 2020 10(1). Read more>>
  • Yu Cui. et al. ACSL4 exacerbates ischemic stroke by promoting ferroptosis-induced brain injury and neuroinflammation. Brain Behav Immun. 2021 JaRead more>>
  • Yuan Wang. et al. Alpha-ketoglutarate ameliorates age-related osteoporosis via regulating histone methylations. Nat Commun. 2020 Nov;11(1):1-14Read more>>
  • Huang Wei. et al. Short-Chain Fatty Acids Ameliorate Diabetic Nephropathy via GPR43-Mediated Inhibition of Oxidative Stress and NF-B Signaling. Oxid Med Cell Longev. 2020;2020:4074832Read more>>
  • Dongmei Yu. et al. Interrod spacing dependent angiogenesis and osseointegration of Na2TiO3 nanorods-patterned arrays via immunoregulation. Chem Eng J. 2021 Jul;:131187Read more>>
  • Shuai Gong. et al. Effects of CRH and ACTH exposure during in?vitro maturation on competence of pig and mouse oocytes. Theriogenology. 2021 Oct;173:211Read more>>
  • Dongmei Yu. et al. Immunomodulation and osseointegration activities of Na2TiO3 nanorods-arrayed coatings doped with different Sr content. Bioact Mater. 2021 SepRead more>>
  • Longfei Xiao. et al. Dihydrotestosterone regulation of cyclooxygenase-2 expression in bovine endometrial epithelium cells by androgen receptor mediated EGFR/PI3K/Akt pathway. J Steroid Biochem. 2021 Nov;214:106001Read more>>
  • Li Yao. et al. OGDHL ameliorates cognitive impairment and Alzheimer's disease-like pathology via activating Wnt/-catenin signaling in Alzheimer's disease mice. Behav Brain Res. 2022 Feb;418:113673Read more>>