bs-0294R-Biotin [Conjugated Secondary Antibody]
Rabbit Anti-Goat IgG Antibody (H+L), Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months..

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:20-100)
IHC-P(1:100-500)
IHC-F(1:50-200)

Cross Reactive Species: Goat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Fei, and YanFen Ye. "Water bath hyperthermia reduces stemness of colon cancer cells." Clinical Biochemistry (2013).Read more>>
  • Wei, Ting, et al. "The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma." Experimental and molecular pathology (2012).Read more>>
  • Liu, Xiao-Yong, et al. "In vitro tissue engineering of lamellar cornea using human amniotic epithelial cells and rabbit cornea stroma." International Journal of Ophthalmology 6.4 (2013): 425.Read more>>
  • Guo, Yonge, et al. "Screening and identification of a specific peptide binding to hepatocellular carcinoma cells from a phage display peptide library." Journal of Peptide Science (2014).Read more>>
  • Guo, Yonge, et al. "Screening and identification of a specific peptide binding to hepatocellular carcinoma cells from a phage display peptide library." Journal of Peptide Science (2014).Read more>>
  • Liu, Qun, et al. "6-Shogaol induces apoptosis in human leukemia cells through a process involving caspase-mediated cleavage of eIF2α." Molecular Cancer 12.1 (2013): 135.Read more>>
  • Ou, Yetao, Jianjie Wang, and Shaobo Zhou. "Antitumor and Apoptosis Induction Effects of Paeonol on Mice Bearing EMT6 Breast Carcinoma." Biomolecules & Therapeutics (구 응용약물학회지) 22.4 (2014): 341-346.Read more>>
  • Wang, Jiawei, et al. "Intravitreal triamcinolone acetonide, retinal microglia and retinal ganglion cell apoptosis in the optic nerve crush model." Acta Ophthalmologica (2015).Read more>>
  • Ji, Mingli, et al. "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits."Experimental and Therapeutic Medicine 12.1 (2016): 51-58.Read more>>
  • Mo, Xian-Gang, et al. "Suppression of NHE1 by small interfering RNA inhibits HIF-1α-induced angiogenesis in vitro via modulation of calpain activity." Microvascular research 81.2 (2011): 160-168.Read more>>
  • Song et al. Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models. (2017) Front.Mol.Neurosc. 10:128Read more>>
  • Xiao et al. An oligodeoxynucleotide with AAAG repeats significantly attenuates burn-induced systemic inflammatory responses via inhibiting interferon regulatory factor 5 pathway. (2017) Mol.Me. 23 Read more>>
  • Lu et al. The osteogenesis-promoting effects of alpha-lipoic acid against glucocorticoid-induced osteoporosis through the NOX4, NF-kappaB, JNK and PI3K/AKT pathways. (2017) Sci.Re. 7:3331Read more>>
  • Zhang et al. A new brominated chalcone derivative suppresses the growth of gastric cancer cells in vitro and in vivo involving ROS mediated up-regulation of DR5 and 4 expression and apoptosis. (2016) Toxicol.Appl.Pharmaco. 309:77-86Read more>>
  • Zhang et al. A novel chalcone derivative S17 induces apoptosis through ROS dependent DR5 up-regulation in gastric cancer cells. (2017) Sci.Re. 7:9873Read more>>
  • Zhang et al. A new brominated chalcone derivative suppresses the growth of gastric cancer cells in vitro and in vivo involving ROS mediated up-regulation of DR5 and 4 expression and apoptosis. (2016) Toxicol.Appl.Pharmaco. 309:77-86Read more>>
  • Zhang et al. A novel chalcone derivative S17 induces apoptosis through ROS dependent DR5 up-regulation in gastric cancer cells. (2017) Sci.Re. 7:9873Read more>>
  • Wang et al. Chlamydia pneumoniae infection promotes vascular smooth muscle cell migration through a Toll-like receptor 2-related signaling pathway. (2013) Infect.Immun. 81:4583-91Read more>>
  • Wan et al. Dietary protein-induced hepatic IGF-1 secretion mediated by PPARγ activation. (2017) PLoS.One. 12:e0173174Read more>>
  • Fiorot RG et al. Synthesis, Molecular Modelling and Anticancer Activities of New Molecular Hybrids Containing 1,4-Naphthoquinone, 7-Chloroquinoline, 1,3,5-Triazine and Morpholine Cores as PI3K and AMPK Inhibitors in the Metastatic Melanoma Cells. J. Braz. Chem. Soc., 2019 00(00):1-14.Read more>>
  • Ma X et al. Fraxin Alleviates LPS-Induced ARDS by Downregulating Inflammatory Responses and Oxidative Damages and Reducing Pulmonary Vascular Permeability. Inflammation. 2019 Jul 4.Read more>>
  • Cao Y et al. Detection of three tumor biomarkers in human lung cancer serum using single particle ICP-MS combined with magnetic immunoassay. Spectrochimica Acta Part B: Atomic Spectroscopy, 105797 (2020). Read more>>
  • Zhu L et al. Role of NDEL1 and VEGF/VEGFR-2 in MouseHippocampus After Status Epilepticus:. ASN Neuro. Jan-Dec 2020;12:1759091420926836.Read more>>
  • Ling-Kun Zhang. et al. Growth factors regional patterned and photoimmobilized scaffold applied to bone tissue regeneration. J Mater Chem B. 2020 Dec;8(48):10990-11Read more>>
  • Huijuan Jin. et al. Screening and identification of a specific peptide binding to breast cancer cells from a phage-displayed peptide library. Biotechnol Lett. 2021 Jan;43(1):153-164Read more>>
  • Huijuan Jin. et al. Screening and identification of a specific peptide binding to breast cancer cells from a phage-displayed peptide library. Biotechnol Lett. 2021 Jan;43(1):153-164Read more>>
  • Linfeng Sun. et al. L-type calcium channel blockers decrease the iron overload-mediated oxidative stress in renal epithelial cells by reducing iron accumulation. Eur J Pharmacol. 2020 Nov;886:173513Read more>>
  • Xiang Yu. et al. Isolation and Identification of Bovine Preadipocytes and Screening of MicroRNAs Associated with Adipogenesis. Animals-Basel. 2020 May;10(5):818Read more>>
  • Zi-Ying Zhan. et al. Taxifolin ameliorate high-fat-diet feeding plus acute ethanol binge-induced steatohepatitis through inhibiting inflammatory caspase-1-dependent pyroptosis. Food Funct. 2021 Jan;12(1):362-372Read more>>
  • Ziteng Deng. et al. Lactobacillus casei protects intestinal mucosa from damage in chicks caused by Salmonella pullorum via regulating immunity and the Wnt signaling pathway and maintaining the abundance of gut microbiota. Poultry Sci. 2021 May;:101283Read more>>
  • Mengfan Chen. et al. SIRT1/PGC-1 signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells. Eur J Pharmacol. 2021 Jun;:174236Read more>>
  • Rui Guo. et al. Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats. Front Neurol. 2021; 12: 54066Read more>>
  • Lei Huang. et al. Changes in the interstitial cells of Cajal in?the?gallbladder of guinea pigs fed a lithogenic diet. Exp Ther Med. 2021 Aug;22(2):1-7Read more>>
  • Li Zhu. et al. Multiple RNA virus matrix proteins interact with SLD5 to manipulate host cell cycle. J Gen Virol. 2021 Dec;102(12):001697Read more>>
  • Sinan Cheng. et al. A novel navigated doxorubicin delivery formulation to breast cancer therapy. MATER TODAY ADV. 2022 Jun;14:100235Read more>>
  • Wangrui Lei. et al. Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling. PHYTOTHER RES. 2022 May 18Read more>>
  • Wei Qin. et al. CAF-derived exosomes transmitted Gremlin-1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenib. MOL CARCINOGEN. 2022 May 31Read more>>
  • Gao, Qian. et al. Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library. BIOTECHNOL LETT. 2022 Jun;:1-1Read more>>
  • Gao, Qian. et al. Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library. BIOTECHNOL LETT. 2022 Jun;:1-1Read more>>
  • Fan, Yucheng. et al. Lysine 222 in PPAR 1 functions as the key site of MuRF2-mediated ubiquitination modification. SCI REP-UK. 2023 Feb;13(1):1-1Read more>>
  • Duo Zeng. et al. FKBP11 improves the malignant property of osteosarcoma cells and acts as a prognostic factor of osteosarcoma. AGING-US. 2023 Apr 15; 15(7): 2450C2459Read more>>
  • Yang Feng. et al. Development a novel drug delivery formulation targeting to esophageal squamous cell carcinoma. MATER TODAY ADV. 2023 Aug;19:100407Read more>>