bs-0295D-HRP [Conjugated Secondary Antibody]
Donkey Anti-Rabbit IgG Antibody (H+L), HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Donkey

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:500-2000)
IHC-P(1:500-2000)
IHC-F(1:500-2000)

Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Qing, et al. "Activation of Autophagy Contributes to the Angiotensin II-Triggered Apoptosis in a Dopaminergic Neuronal Cell Line." Molecular Neurobiology (2015): 1-9.Read more>>
  • Zhang, Jingjing, et al. "Probing cell–matrix interactions in RGD-decorated macroporous poly (ethylene glycol) hydrogels for 3D chondrocyte culture."Biomedical Materials 10.3 (2015): 035016.Read more>>
  • Kashiwabara, Yoshiaki, et al. "Immunohistochemical localization of Nox in mouse circumvallate papillae." Tissue and Cell (2015).Read more>>
  • Qin, Sida, et al. "Fibronectin protects lung cancer cells against docetaxel-induced apoptosis by promoting Src and caspase-8 phosphorylation." Tumor Biology (2016): 1-12.Read more>>
  • Maurer, Susanne, et al. "Tasting Pseudomonas aeruginosa biofilms: human neutrophils express the bitter receptor T2R38 as sensor for the quorum sensing molecule N-(3-oxododecanoyl)-l-homoserine lactone." Frontiers in Immunology 6 (2015).Read more>>
  • Qin, Sida, et al. "XIAP inhibits mature Smac-induced apoptosis by degrading it through ubiquitination in NSCLC." International Journal of Oncology.Read more>>
  • Zhang, Jingjing, et al. "Physically entrapped gelatin in polyethylene glycol scaffolds for three-dimensional chondrocyte culture." Journal of Bioactive and Compatible Polymers: Biomedical Applications (2016): 0883911516633893.Read more>>
  • Wang, Jun, et al. "Expression of Talin1 in tissues of ovarian cancer and its role in invasion and migration of ovarian cancer cells." Int J Clin Exp Med 10.1 (2017): 410-417.Read more>>
  • Xiang, Sen, et al. "ASS1 expression in liver cancer tissues and effects of arginine deprivation on invasion and migration of liver cancer cells." Int J Clin Exp Med 10.2 (2017): 2469-2477.Read more>>
  • Song, Zhiqi, et al. "REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling." Oncotarget 7.11 (2016): 12035.Read more>>
  • Gao, Xiaoying, et al. "Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway." Molecular Medicine Reports.Read more>>
  • Zhang, Yu, et al. "JAK-STAT signaling regulation of chicken embryonic stem cell differentiation into male germ cells." In Vitro Cellular & Developmental Biology-Animal (2017): 1-16.Read more>>
  • Song et al. Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models. (2017) Front.Mol.Neurosc. 10:128Read more>>
  • Chen et al. Functional roles of C/EBPα and SUMO‑modification in lung development. (2017) Int.J.Mol.Me. 40:1037-1046Read more>>
  • Gao et al. Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. (2017) Mol.Med.Rep. 16:515-522Read more>>
  • Hu et al. MARCH5 RNA promotes autophagy, migration, and invasion of ovarian cancer cells. (2017) Autophagy. 13:333-344Read more>>
  • Chen,et al.Mitochondrial ATAD3A regulates milk biosynthesis and proliferation of mammary epithelial cells from dairy cow via the mTOR pathway.(2018) Cell Biology International. 42:533-542.Read more>>
  • Ding,et al.Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice.(2018) Immunity & Ageing. 15:6.Read more>>
  • Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25.Read more>>
  • Lv LL et al. Exosomal miRNA-19b-3p of tubular epithelial cells promotes M1 macrophage activation in kidneyinjury. Cell Death Differ. 2019 May 16. Read more>>
  • Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852.Read more>>
  • Gao J et al. Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep.Animals (Basel). 2019 May 31;9(6). pii: E309.Read more>>
  • Gu L et al. Dimethyl itaconate protects against fungal keratitis by activating the Nrf2/HO‐1 signaling pathway. Immunol Cell Biol. 2020 Jan 13.Read more>>
  • Xu T et al. Elevated mRNA expression levels of DLGAP5 are associated with poor prognosis in breast cancer. Oncol Lett. 2020 Jun;19(6):4053-4065.Read more>>
  • Ye Fenget al. Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. Cell Death Dis. 2020 Jul 8;11(7):513.Read more>>
  • Hu A et al. Ginsenoside Rg1 prevents vascular intimal hyperplasia involved by SDF-1/CXCR4, SCF/c-kit and FKN/CX3CR1 axes in a rat balloon injury. J Ethnopharmacol. 2020 Oct 5;260:113046.Read more>>
  • Zhang NN et al. Fam70A binds Wnt5a to regulate meiosis and quality of Mouseoocytes. Cell Prolif. 2020 Jun;53(6):e12825.Read more>>
  • Wen-Jun Gan. et al. Kuwanon G protects HT22 cells from advanced glycation end product-induced damage. Exp Ther Med. 2021 May;21(5):1-9Read more>>
  • Yu Cui. et al. ACSL4 exacerbates ischemic stroke by promoting ferroptosis-induced brain injury and neuroinflammation. Brain Behav Immun. 2021 JaRead more>>
  • Fei Wu. et al. Enhanced targeted delivery of adenine to hepatocellular carcinoma using glycyrrhetinic acid-functionalized nanoparticles in vivo and in vitro. Biomed Pharmacother. 2020 Nov;131:110682Read more>>
  • Huang Wei. et al. Short-Chain Fatty Acids Ameliorate Diabetic Nephropathy via GPR43-Mediated Inhibition of Oxidative Stress and NF-B Signaling. Oxid Med Cell Longev. 2020;2020:4074832Read more>>
  • Chunyu Jiang. et al. AMD3100 and SDF?1 regulate cellular functions of endothelial progenitor cells and accelerate endothelial regeneration in a rat carotid artery injury model. Mol Med Rep. 2020 Oct;22(4):3201-3212Read more>>
  • Dongmei Yu. et al. Interrod spacing dependent angiogenesis and osseointegration of Na2TiO3 nanorods-patterned arrays via immunoregulation. Chem Eng J. 2021 Jul;:131187Read more>>
  • Dongmei Yu. et al. Immunomodulation and osseointegration activities of Na2TiO3 nanorods-arrayed coatings doped with different Sr content. Bioact Mater. 2021 SepRead more>>